FAQ's of Human CFH(Complement Factor H) ELISA Kit:
Q: How do I perform the Human CFH ELISA assay with this kit?
A: Begin by adding 50-100 l of your prepared sample, standard, or control to the pre-coated 96-well plate, following the instruction manual. Incubate, wash, and sequentially add detection antibody, HRP conjugate, substrate, and stop solution. Finally, measure absorbance at 450 nm using an ELISA microplate reader to quantify CFH concentration.
Q: What sample types are compatible with this ELISA kit?
A: The Human CFH ELISA Kit supports serum, plasma, cell culture supernatant, and a variety of biological fluids, offering flexibility for different experimental needs in bioscience research.
Q: When should the kit components be stored at 2-8C or -20C?
A: Store the entire kit at 2-8C upon receipt, except for the standard and concentrated solutions, which require storage at -20C for maximum stability and shelf-life (6-12 months). For detailed guidance, refer to the instruction manual provided.
Q: Where can I use this kit and what equipment do I need?
A: This kit is intended for research use in laboratory settings, specifically suited for distributors, importers, and suppliers in India. It is fully compatible with standard ELISA microplate readers operating at 450 nm.
Q: What are the major benefits and features of the Human CFH ELISA Kit?
A: The kit provides highlighted sensitivity (<9.38 ng/ml), classic double-antibody sandwich specificity, and supreme reproducibility (<10% CV). Imposing purity (>98%) and a comprehensive reagent set ensure accurate, reliable results for human CFH quantification, assisting high-standard laboratory workflows.