FAQ's of Human CLU(Clusterin) ELISA Kit:
Q: How is the Human CLU (Clusterin) ELISA Kit utilised in research?
A: The kit is used for quantitative analysis of Clusterin in human serum, plasma, and cell culture supernatant, typically for biomedical or clinical research applications.
Q: What is the main detection method employed in this ELISA Kit?
A: It employs a Sandwich ELISA format, utilising an HRP-conjugated antibody and colorimetric detection at 450 nm for accurate quantification.
Q: Where can the kit be used besides human samples?
A: While designed for human samples, the kit may be applicable to other biological samples such as cell culture supernatants, provided they contain human clusterin.
Q: What is the process for running a test with this kit?
A: Add standards and samples to the pre-coated plate, incubate with HRP conjugate, wash, add substrate and stop solution, and measure absorbance at 450 nm using a microplate reader.
Q: What benefits does this kit offer over standard assays?
A: The kit offers superior sensitivity (as low as 0.056 ng/mL), wide assay range, and high reproducibility, ensuring accurate and reliable results for critical research.
Q: When should the kit be stored, and at what temperature?
A: The kit should be stored immediately upon receipt at 2-8C to maintain reagent stability with a shelf life of 6-12 months.