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Human Dipeptidyl Peptidase ELISA Kit

Human Dipeptidyl Peptidase  ELISA Kit
Human Dipeptidyl Peptidase ELISA Kit
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Product Code : SRGHE0011
Brand Name : SR GROUP ELISA
Price And Quantity
  • Minimum Order Quantity
  • 1
  • Unit of Measure
  • Pack/Packs
  • Price
  • 17500-19800 INR
  • Price Range
  • 17500.00 - 19800.00 INR
Product Description

SAMPLE TYPE- serum, plasma and other biological fluids.

Assaytype             Sandwich

Format                   96T

Assaytime             3.5h

Reactivity               Human

DetectionMethod               Colorimetric

DetectionRange          0.31~20 ng/ml

Sensitivity                   0.19ng/mL

SampleVolume Required Per Well  100ul


Kitcomponents & Storage

Item

Specifications

Micro ELISA Plate(Dismountable)

8 wells X 12 strips

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100x)

1 vial, 120 ul

Concentrated HRP Conjugate (100x)

1 vial, 120 ul

Reference Standard & Sample Diluent

1 vial, 20 mL

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25x)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

Stop Solution

1 vial, 10 mL





TEST PROCEDURE

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

 

2. Remove the liquid.

 

3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

 

4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

 

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

 

6.Add 50 μL Stop Solution.

           7. Read at 450 nm immediately. Calculation of results

 

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Contact no-011-27511805, Mobile-+91-9990521622


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