FAQ's of Human E-Cad(E-Cadherin) ELISA Kit:
Q: How is the Human E-Cad (E-Cadherin) ELISA Kit used in research?
A: The kit is used for precise quantification and qualification of E-Cadherin levels in human biological fluids such as serum, plasma, urine, and tissue homogenates using a reliable sandwich ELISA method.
Q: What is the detection range and sensitivity of this ELISA kit?
A: The assay offers a detection range of 0.32-20 ng/mL and an ultra-sensitive detection limit of approximately 0.1 ng/mL, according to lot-specific datasheets.
Q: When should the kit be stored, and at what temperature?
A: For maximum shelf life (6-12 months), kits should be stored immediately upon receipt at 2-8C, and repeated freeze-thaw cycles should be avoided.
Q: Where can the Human E-Cadherin ELISA Kit be applied?
A: It is suitable for research use in clinical, diagnostic, and academic laboratories, particularly for studying cell adhesion and related processes in human samples.
Q: What is the process for running an assay with this kit?
A: After sample preparation, add 50-100 L per well to the pre-coated microplate, follow the stepwise protocol involving standard, detection reagent, enzyme conjugate, wash buffer, substrate, and stop solution, all included in the kit.
Q: What benefits does the kit offer to researchers?
A: It provides high precision (CV < 10%), robust linearity, no significant cross-reactivity, pre-packaged convenience, and clear directions, delivering accurate, reproducible results at an unbeatable price.