FAQ's of Human HRH2(Histamine H2 receptor) ELISA Kit:
Q: How does the Human HRH2 ELISA Kit detect the target protein?
A: The kit utilizes the sandwich ELISA principle: samples containing HRH2 antigen are captured on a pre-coated plate. Detection antibodies bind specifically, followed by HRP-conjugate and TMB substrate for colorimetric detection. The signal corresponds to the target concentration.
Q: What is the typical assay duration for this kit?
A: The assay procedure generally takes between 2 to 4 hours, with most protocols completed within approximately 3.5 hours, allowing for efficient workflow in laboratory settings.
Q: What sample types can be analyzed using this kit?
A: Compatible sample types include human serum, plasma, cell culture supernatants, tissue homogenates, cell lysates, and other biological fluids, ensuring broad applicability across research projects.
Q: Where should the ELISA kit be stored for optimal stability?
A: Store the kit at 2-8C and avoid exposure to light for its full shelf life of 6-12 months. Proper storage preserves reagent activity and assay reliability.
Q: What is the benefit of using this kit over others?
A: This ELISA Kit stands out due to its best-in-class price promotion, high sensitivity (down to 0.06 ng/mL), superior precision (intra-assay CV <8%), and a comprehensive range of included components for reliable, reproducible results in research applications.