FAQ's of Human IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit:
Q: How does the Human IGFBP4 ELISA Kit work?
A: The kit uses a sandwich ELISA principle where IGFBP4 in your sample binds to a capture antibody pre-coated on a microplate. It's then detected by a biotinylated antibody and HRP-streptavidin, followed by colorimetric detection, which enables quantification.
Q: What sample types are compatible with this ELISA kit?
A: You can use serum, plasma, cell culture supernates, tissue homogenates, cell lysates, and other human biological fluids for this assay, ensuring broad application utility.
Q: When should the reagents from the kit be used after opening?
A: Reagents should be used promptly after opening. Always store unused components at 2-8C and do not freeze reagents, as per the instruction manual, to maintain kit integrity throughout its shelf life (6-12 months).
Q: Where can I use this kit and for what research purposes?
A: This kit is intended solely for research use and not for diagnostic or therapeutic applications. It's suitable for biomedical research, clinical studies, and cell biology investigations related to IGFBP4 quantification.
Q: What is the sample volume required for each well?
A: Typically, 50-100 L per well is needed for analysis, as indicated in the kit's datasheet. Ensure to follow precise protocol for optimal results.
Q: How does this kit benefit researchers?
A: It delivers reliable, quantitative results of IGFBP4 with high sensitivity and reproducibility, supporting advanced understanding and discovery in metabolic, growth, or cancer-related pathways that involve IGFBP4.