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Human Placental Protein 14 ELISA KIT

Human Placental Protein 14 ELISA KIT
Human Placental Protein 14 ELISA KIT
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Product Code : srgche005
Brand Name : S.R GROUP
Price And Quantity
  • Minimum Order Quantity
  • 1 Pack
  • Price
  • 17500-19800 INR
  • Price Range
  • 17500.00 - 19800.00 INR
Product Description

  PlacentalProtein 14 ELISA KIT     

ThisELISA kit applies to the in vitro quantitative determination of Human protein14  concentrations in serum, plasma and otherbiological fluids.

Assaytype             Sandwich

Format                   96T

Assaytime             3.5h

Reactivity               Human

DetectionMethod               Colorimetric

DetectionRange           4.69-300ng/ml

Sensitivity                    2.81ng/ml

Kitcomponents & Storage

Item

Specifications

Micro ELISA Plate(Dismountable)

8 wells ×12 strips

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100×)

1 vial, 120 μL

Concentrated HRP Conjugate (100×)

1 vial, 120 μL

Reference Standard & Sample Diluent

1 vial, 20 mL

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25×)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

Stop Solution

1 vial, 10 mL

Plate Sealer

5 pieces

Product Description

1 copy

Certificate of Analysis

1 copy

TEST PROCEDURE

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

 

2. Remove the liquid.

 

3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

 

4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

 

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

 

6.Add 50 μL Stop Solution.

           7. Read at 450 nm immediately. Calculation of results

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