FAQ's of Human SELE(Selectin, Endothelium) ELISA Kit:
Q: How does the Human SELE ELISA Kit ensure accurate detection of E-Selectin?
A: The kit uses a polished sandwich ELISA principle based on antigen-antibody specificity, combined with HRP colorimetric detection. High-quality components and a provided standard curve guarantee reliable results within the 15.6-1000 pg/mL detection range, with strong batch-to-batch precision controls.
Q: What sample types can be analyzed using this ELISA kit?
A: The Human SELE (Selectin, Endothelium) ELISA Kit is compatible with serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids, offering researchers grand flexibility in their analysis.
Q: When should the kit be stored, and under what conditions?
A: To preserve superior kit performance, store all components at 2-8C and avoid freeze/thaw cycles. The shelf life ranges from 6 to 12 months, ensuring longevity for your research projects.
Q: Where is the Human SELE ELISA Kit shipped from, and how is it packaged?
A: All orders are dispatched from trusted Indian FOB ports. Kits are securely packaged, featuring a pre-coated 96-well microplate and individual reagent vials, to safeguard quality during transit.
Q: What is the process for running a SELE ELISA assay?
A: Add 100 L of your biological sample to each well, incubate with detection antibody and HRP conjugate, then follow with substrate and stop solution steps. Measure absorbance at 450 nm for quantitative results using the standard curve supplied.
Q: How can using this kit benefit my research?
A: This kit delivers rare, research-grade accuracy, rapid operation (approx. 3.5 hours), and validated sensitivity. It empowers comprehensive analysis of endothelial activation, benefiting vascular biology and immunology investigations.