Product Description
| Reactivity: |
Human |
| Alternative Names: |
Major synaptic vesicle protein p38 |
| Assay Type: |
Sandwich |
| Sensitivity: |
10.34 pg/mL |
| Standard: |
2000 pg/mL |
| Detection Range: |
31.25-2000 pg/mL |
| Sample Type: |
Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: |
3.5h |
Precise Quantification of Human SynaptophysinThis ELISA kit was developed for the specific and quantitative detection of synaptophysin in human biological samples, facilitating accurate measurement for neuroscience and biomarker studies. The sandwich ELISA format provides exceptional sensitivity and linearity, backed by stringent calibration standards, ensuring reliable results essential for research projects.
Comprehensive and Convenient Kit DesignEach kit contains all necessary reagents for immediate use, including pre-coated plates, ready-to-use HRP conjugate, standards, buffers, and substrates. The kit accommodates a 96-well microplate format, supporting moderate throughput and efficient sample processing. Simply follow the clear, stepwise protocol for optimal performance.
Built for Research Flexibility and SafetySuitable for a range of applications in research settings, the kit is non-hazardous and odorless, requiring standard laboratory precautions. Components should be stored at 2-8C for optimal stability and shelf life, with no freezing necessary unless otherwise specified in the included instructions.
FAQ's of Human SYP(Synaptophysin) ELISA Kit:
Q: How does the Human SYP (Synaptophysin) ELISA Kit detect synaptophysin in samples?
A: The kit employs a sandwich ELISA format, where human synaptophysin in your sample binds to antibodies coated on the microplate, followed by a second antibody linked to an enzyme. This forms an antibody-antigen-antibody complex, which generates a color signal proportional to synaptophysin concentration during the color development step.
Q: What is the recommended sample volume for each test well?
A: The assay is optimized for a sample volume of 50-100 L per well. This allows for precise detection within the specified concentration range. Please follow the protocol for accurate measurements.
Q: When should I use this kit for my research?
A: This ELISA kit is designed for the quantitative measurement of synaptophysin in human samples, making it ideal for neuroscience research, biomarker studies, and projects requiring reliable synaptophysin quantification. Use it when precise, reproducible data is essential.
Q: Where can calibration and standard curve information be found?
A: Calibration standards and instructions for generating the standard curve are included within the kit. The standard curve enables you to accurately calculate synaptophysin concentrations in your test samples.
Q: What is the assay process and how long does it take to complete?
A: The entire assay process typically takes 2-4 hours, including sample incubation, addition of detection antibodies and substrates, and color development. A step-by-step protocol is provided with every kit to guide you through the procedure efficiently.
Q: Can I store the kit components at room temperature?
A: No. For best results and to preserve reagent activity, store all kit components at 2-8C. Do not freeze any components unless specified in the protocol.
Q: What are the main benefits of using this ELISA kit?
A: The Human SYP ELISA Kit offers high specificity, sensitivity (<0.1 ng/mL), and excellent reproducibility, with ready-to-use components for efficient workflow. It supports robust quantification of synaptophysin in human samples, facilitating research advancements with reliable data.