FAQ's of Human TERT(Telomerase Reverse Transcriptase) ELISA Kit:
Q: How does the Human TERT ELISA Kit work?
A: The kit utilizes a sandwich ELISA approach, where a microplate pre-coated with antibodies specific for hTERT captures the target protein. Detection is carried out using enzyme-conjugated secondary antibodies and a colorimetric substrate, with quantification based on absorbance at 450 nm.
Q: What types of samples can I analyze with this kit?
A: You can use human serum, plasma, tissue homogenates, and cell culture supernatants. It is designed for a wide application in quantifying TERT in various biological fluids and tissue samples.
Q: Where should the kit be stored for optimal stability?
A: Store the Human TERT ELISA Kit at 2-8C and protect it from light to maintain the stability and efficacy of the reagents throughout its 6-12 month shelf life.
Q: What is the process for performing the assay?
A: Add your samples to the pre-coated wells, incubate with detection antibody and enzyme conjugate, add substrate, and stop solution, then measure absorbance at 450 nm with a microplate reader. The total assay time is approximately 3.5-4 hours.
Q: What are the material and safety features of this kit?
A: The kit contains high-purity reagents (>98%), is non-poisonous, odorless, and free from hazardous chemicals. It follows standard laboratory safety practices and is classified as Research Use Only (RUO).