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Human Tyrosine Kinase 2 ELISA KIT

Human Tyrosine Kinase 2 ELISA KIT
Human Tyrosine Kinase 2 ELISA KIT
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Product Code : srgche004
Brand Name : S.R GROUP
Price And Quantity
  • Minimum Order Quantity
  • 1
  • Unit of Measure
  • Pack/Packs
  • Price
  • 17500-19800 INR
  • Price Range
  • 17500.00 - 19800.00 INR
Product Description

Tyrosine Kinase 2 ELISA KIT     

ThisELISA kit applies to the in vitro quantitative determination of Human kinase 2 concentrationsin serum, plasma and otherbiological fluids.

Assay type          Sandwich

Format                 96T

Assay time          3.5h

Reactivity            Human

Detection Method          Colorimetric

Detection Range           0.31-20ng/ml

Sensitivity                    0.19ng/ml

Sample Volume Required Per Well          100μL

Kitcomponents & Storage

Item

Specifications

Micro ELISA Plate(Dismountable)

8 wells ×12 strips

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100×)

1 vial, 120 μL

Concentrated HRP Conjugate (100×)

1 vial, 120 μL

Reference Standard & Sample Diluent

1 vial, 20 mL

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25×)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

Stop Solution

1 vial, 10 mL

Plate Sealer

5 pieces

Product Description

1 copy

Certificate of Analysis

1 copy

TEST PROCEDURE

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

 

2. Remove the liquid.

 

3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

 

4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

 

5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

 

6.Add 50 μL Stop Solution.

           7. Read at 450 nm immediately. Calculation of results

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