FAQ's of Mouse CLU(Clusterin) ELISA Kit:
Q: How does the Mouse CLU (Clusterin) ELISA Kit work?
A: The kit utilizes a sandwich ELISA principle where Clusterin from mouse samples is specifically captured by pre-coated antibodies, then detected using HRP-conjugated antibodies and a colorimetric substrate for quantification at 450 nm.
Q: What sample types are compatible with this ELISA kit?
A: This kit is validated for mouse serum, plasma, tissue homogenates, cell lysates, and other biological fluids, providing broad applicability for researchers.
Q: When should I use this kit in my research?
A: Use this kit when precise quantification of Clusterin (ApoJ, CLU) is required in mouse samples, particularly in projects focused on cell biology, neuroscience, or immunology.
Q: Where should the kit be stored upon arrival?
A: Store the kit at 2-8C immediately after delivery. Avoid repeated freeze-thaw cycles to protect reagent integrity.
Q: What is the process for running the assay?
A: The process takes 2-4 hours and involves sample incubation with pre-coated plates, successive addition of detection reagents, HRP-conjugate, substrate, and reading absorbance at 450 nm as per the instruction manual.
Q: What benefits does this kit offer for laboratory research?
A: The kit delivers high precision and sensitivity (<10 pg/mL), includes all assay components, and provides reproducible results, making it valuable for quantitative CLU studies in mouse models.