FAQ's of Mouse ErbB3(V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit:
Q: How is the Mouse ErbB3 ELISA Kit typically used in research?
A: The Mouse ErbB3 ELISA Kit is applied for quantitative detection of ErbB3 protein in various biological samples, such as serum, plasma, cell culture supernatants, and tissue homogenates, mainly in the context of biomedical and oncology research.
Q: What is included in the kit and how much sample is required per test?
A: The kit provides a pre-coated 96-well microplate, detection and standard reagents, substrates, wash buffer, stop solution, instruction manual, and more. Each assay requires 50-100 l of sample per well.
Q: When should the Mouse ErbB3 ELISA Kit be used, and what is its assay duration?
A: The kit is suitable for projects requiring sensitive, specific ErbB3 quantification in mouse samples. The total assay takes approximately 2-3 hours, ensuring quick results for efficient workflow.
Q: Where can this ELISA kit be stored and how long is its shelf life?
A: The ELISA Kit should be stored at 2-8C and has a shelf life of 6-12 months, provided freeze-thaw cycles are avoided to maintain reagent integrity.
Q: What is the process for running the ELISA assay?
A: The assay process involves adding standards and samples to the wells, followed by sequential addition of detection reagents and substrates. After incubation and washing steps, the reaction is measured at 450 nm using a microplate reader.
Q: What benefits does this kit provide over other available options?
A: This kit stands out due to its exceptional sensitivity (<0.1 ng/ml), broad detection range, detailed instruction manual, high purity, and QC-tested accuracy, offering researchers dependable results for protein quantification.