FAQ's of Mouse HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit:
Q: How does the Mouse HCAM ELISA Kit ensure high sensitivity and specificity in detection?
A: This kit utilises a high-affinity Sandwich ELISA format with HRP-conjugated detection, achieving a sensitivity of 0.061 ng/mL. Optimised reagents and stringent quality control guarantee specific and accurate quantification of HCAM in mouse samples.
Q: What sample types can be analysed using this ELISA Kit?
A: You can utilise this kit to analyse serum, plasma, cell culture supernatants, tissue homogenates, and other mouse biological fluids, offering versatility for various experimental protocols.
Q: When will the kit typically be delivered after procurement?
A: Following confirmation of the order and payment terms, the kit is promptly dispatched with delivery time depending on your location within India. Most drop-off times are communicated at the point of sale for transparency.
Q: Where should the Mouse HCAM ELISA Kit be stored for optimal stability?
A: Store the kit at 2-8C in a refrigerator. Avoid exposure to direct sunlight and repeated freeze/thaw cycles to maintain assay integrity throughout its shelf life of 6-12 months.
Q: What is the process for preparing and running the assay?
A: Detailed instructions are included in the kit. Briefly, add samples and standards to the pre-coated plate, then proceed through incubation, washing, and the addition of HRP-conjugated detection reagent, substrate, and stop solution before reading at 450 nm.
Q: Can the kit components be re-used for multiple assays?
A: No, the kit components are designed for single-use applications to ensure precision and avoid cross-contamination. Use each reagent and the pre-coated plate as specified in the included manual.