FAQ's of Mouse KNG1(Kininogen 1) ELISA Kit:
Q: How is the Mouse KNG1 (Kininogen 1) ELISA Kit used in research?
A: This kit is utilized for the quantitative measurement of Mouse Kininogen 1 in serum, plasma, or cell culture supernates, providing precise and reliable data for research in immunology and related biomedical fields.
Q: What is the process of running the Mouse KNG1 ELISA Kit?
A: The kit follows a sandwich ELISA protocol: add your prepared samples to the 96-well pre-coated microplate, follow with standards, biotin-antibody, HRP-avidin, and then substrate. After specified incubation times, read the results at 450 nm using a microplate reader.
Q: When should all reagents of the kit be brought to room temperature?
A: All reagents must be brought to room temperature before beginning the assay to ensure optimal reaction conditions and accurate, consistent results.
Q: Where can the Mouse KNG1 ELISA Kit be stored and for how long?
A: Store the kit at 2-8C (standard refrigeration). The shelf life ranges from 6 to 12 months under proper storage conditions. Do not freeze the reagents.
Q: What are the main benefits of using this ELISA kit?
A: Researchers gain high sensitivity (min detectable <14 pg/mL), 98% specificity, and precise, reproducible results, making it ideal for detecting KNG1 levels in mouse samples with minimal cross-reactivity issues.
Q: How does the kit ensure high precision in results?
A: The kit's intra-assay coefficient of variation is less than 10%, and inter-assay CV is less than 12%, supporting high data reliability and reproducibility.