FAQ's of Mouse KRT19(Keratin 19) ELISA Kitc:
Q: How should the Mouse KRT19 (Keratin 19) ELISA Kitc be stored to ensure optimal performance?
A: Store the kit at 2-8C and avoid repeated freeze/thaw cycles. Proper storage will help maintain reagent stability and assay sensitivity throughout the stated 6-12 month shelf life.
Q: What process should I follow for using the Mouse KRT19 ELISA Kitc?
A: Begin by preparing your samples and standards, then add them to the pre-coated microplate wells. Follow with detection reagents, incubate as directed, and measure the colorimetric signal at 450 nm using a microplate reader. Detailed protocols are provided in the instruction manual.
Q: When can I expect results after running the assay?
A: The entire assay process typically takes about 2-3.5 hours from sample addition to result reading, depending on incubation settings and operator efficiency.
Q: Where can this kit be applied in research settings?
A: This ELISA Kit is suitable for molecular biology, oncology, and clinical research labs requiring precise quantification of KRT19 in mouse serum, plasma, or biological fluids.
Q: What is the main benefit of using the Mouse KRT19 ELISA Kitc in experiments?
A: The kit offers phenomenal sensitivity and specificity, enabling researchers to detect minute levels of Keratin 19 with high accuracy and reproducibility, especially useful in disease marker studies and experimental analysis.
Q: How many samples can be tested per plate, and what volume is required?
A: Each kit contains a 96-well microplate, which allows testing of up to 88 samples, depending on the number of standards and controls. Typically, each well requires 50-100 L of sample.