Mouse ME20M(Melanoma Associated ME20) ELISA Kit
Mouse ME20M(Melanoma Associated ME20) ELISA Kit
Mouse ME20M(Melanoma Associated ME20) ELISA Kit
Mouse ME20M(Melanoma Associated ME20) ELISA Kit
Mouse ME20M(Melanoma Associated ME20) ELISA Kit
Mouse ME20M(Melanoma Associated ME20) ELISA Kit

Mouse ME20M(Melanoma Associated ME20) ELISA Kit

Price 25750.0 INR/ Piece

MOQ : 1 Piece

Mouse ME20M(Melanoma Associated ME20) ELISA Kit Specification

  • Smell
  • Odorless
  • Usage
  • RESEARCH
  • Other Names
  • ME20M ELISA Kit
  • HS Code
  • 3822.00
  • Physical Form
  • Kit contains both solid and liquid components
  • Shelf Life
  • 6-12 Months
  • Shape
  • Solid (Microplate), Liquid (Buffers/Reagents)
  • Poisonous
  • No (For research use only, not for human or animal therapeutic use)
  • Storage
  • 2-8C, protect from light, do not freeze reagents
  • Appearance
  • Kit with pre-coated microplate, reagents, and controls
  • Application
  • Quantitative measurement of ME20M in mouse serum, plasma, or tissue homogenates
  • Grade
  • Research Grade
  • Ph Level
  • 7.2-7.4 (Assay buffer)
  • Classification
  • Immunoassay Reagent
  • Chemical Name
  • Mouse ME20M (Melanoma Associated ME20)
  • Type
  • ELISA Kit
  • Purity(%)
  • >98% (as determined by SDS-PAGE)
  • Color
  • Clear/Colorless (Reagents), White (96-well plate)
  • Assay Range
  • 15.6-1000 pg/mL
  • Incubation Time
  • 2-4 hours (total)
  • Detection Method
  • Sandwich ELISA, colorimetric detection
  • Sensitivity
  • <10 pg/mL
  • Microplate Type
  • 96-well strip plate, pre-coated with antibody
  • Controls Included
  • Positive and negative controls provided
  • Safety Precautions
  • Handle all kit components using standard laboratory precautions, not for human diagnostic or therapeutic use
  • Reproducibility
  • Intra- and Inter-assay CV <10%
  • Kit Contents
  • Pre-coated microplate, standards, sample diluent, wash buffer, detection antibody, substrate, stop solution, instruction manual
  • Species Reactivity
  • Mouse
  • Standard Curve
  • Provided in kit
  • Limit of Detection
  • Less than 10 pg/mL
  • Precision
  • High
  • Sample Volume Required
  • 50 L per well
 

Mouse ME20M(Melanoma Associated ME20) ELISA Kit Trade Information

  • Minimum Order Quantity
  • 1 Piece
  • Supply Ability
  • 500 Pieces Per Day
  • Delivery Time
  • 1-7 Days
 

About Mouse ME20M(Melanoma Associated ME20) ELISA Kit

  1. Reactivity: Mouse
    Alternative Names: PMEL; GP100; SI; SIL; SILV; Pmel17; Silver Homolog; Premelanosome Protein; Silver Locus Protein Homolog; 95 kDa melanocyte-specific secreted glycoprotein
    Assay Type: Sandwich
    Sensitivity: 0.057 ng/mL
    Standard: 10 ng/mL
    Detection Range: 0.16-10 ng/mL
    Sample Type: tissue homogenates, cell lysates and other biological fluids
    Assay Length: 3.5h


High Sensitivity and Precise Quantification

With a detection sensitivity below 10 pg/mL and an assay range from 15.6 to 1000 pg/mL, the Mouse ME20M ELISA Kit delivers outstanding accuracy for low-abundance protein detection. High intra- and inter-assay precision (CV <10%) ensures reproducible results, making this kit ideal for demanding research applications focused on melanoma-associated ME20 in mouse samples.


User-Friendly Format for Streamlined Workflow

Designed for convenience, the kit is supplied with a pre-coated 96-well strip plate and all essential reagents. Positive and negative controls, as well as standards for the standard curve, are included. The protocol requires only a modest 50 L of sample per well and can be completed within 2-4 hours, optimizing laboratory productivity.


Reliable Results for Diverse Sample Types

This ELISA kit is compatible with mouse serum, plasma, and tissue homogenates. It is carefully designed to maintain the integrity of research results, with a shelf life of 6-12 months provided it is stored at 2-8C and protected from light. All components are odourless and non-toxic for safe lab handling. This research-grade kit is for laboratory use only.

FAQ's of Mouse ME20M(Melanoma Associated ME20) ELISA Kit:


Q: How is the ME20M concentration quantified using this ELISA kit?

A: Quantification is performed through a sandwich ELISA method, where a 96-well plate pre-coated with ME20M-specific antibody captures the target protein from the sample. Bound ME20M is detected using an enzyme-conjugated antibody and a colorimetric substrate. The absorbance produced is proportional to ME20M concentration and is quantified by reference to the provided standard curve.

Q: What sample types can be analyzed with this kit and what is the required volume?

A: The kit is validated for mouse serum, plasma, and tissue homogenates. Only 50 L of each sample is needed per well, enabling efficient use of limited or precious biological materials.

Q: When should reagents and samples be prepared for best results?

A: Prepare all reagents, standards, and samples immediately before starting the assay, following the protocols in the instruction manual. To preserve reagent integrity, keep components at 2-8C, avoid light exposure, and do not freeze liquid reagents.

Q: Where should the Mouse ME20M ELISA Kit be stored?

A: Store the entire kit and its components at 2-8C and protect from light. Do not freeze liquid reagents, as this may compromise their functionality. Proper storage extends the shelf life up to 12 months.

Q: What quality controls are included to ensure reliable results?

A: Both positive and negative controls are supplied in the kit. These controls validate the assay's accuracy, performance, and reproducibility every time the assay is performed.

Q: How long does the assay take, and what is the main advantage?

A: The complete assay can be performed within 2 to 4 hours. Its main advantage is a sensitive, quantitative readout of ME20M in mouse samples with high precision and reproducibility, ideal for research applications.

Q: What are the benefits of using this ELISA kit for research?

A: This kit offers sensitive and precise measurement of ME20M, convenient workflow with pre-measured reagents, and compatibility with multiple sample types. Its rigorous quality controls and straightforward protocol make it a reliable choice for quantitative research on melanoma-associated markers in mice.

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