Mouse ME20M(Melanoma Associated ME20) ELISA Kit
25750.0 INR/Piece
Product Details:
- Usage RESEARCH
- Shape Solid (Microplate), Liquid (Buffers/Reagents)
- Grade Research Grade
- HS Code 3822.00
- Appearance Kit with pre-coated microplate, reagents, and controls
- Application Quantitative measurement of ME20M in mouse serum, plasma, or tissue homogenates
- Storage 2-8C, protect from light, do not freeze reagents
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Mouse ME20M(Melanoma Associated ME20) ELISA Kit Price And Quantity
- 25750.0 INR/Piece
- 1 Piece
Mouse ME20M(Melanoma Associated ME20) ELISA Kit Product Specifications
- ME20M ELISA Kit
- No (For research use only, not for human or animal therapeutic use)
- 7.2-7.4 (Assay buffer)
- Clear/Colorless (Reagents), White (96-well plate)
- 6-12 Months
- Mouse ME20M (Melanoma Associated ME20)
- Kit contains both solid and liquid components
- Odorless
- RESEARCH
- ELISA Kit
- Solid (Microplate), Liquid (Buffers/Reagents)
- >98% (as determined by SDS-PAGE)
- Immunoassay Reagent
- 3822.00
- Research Grade
- Quantitative measurement of ME20M in mouse serum, plasma, or tissue homogenates
- 2-8C, protect from light, do not freeze reagents
- Kit with pre-coated microplate, reagents, and controls
- Less than 10 pg/mL
- 96-well strip plate, pre-coated with antibody
- Sandwich ELISA, colorimetric detection
- <10 pg/mL
- 50 L per well
- Handle all kit components using standard laboratory precautions, not for human diagnostic or therapeutic use
- Mouse
- Provided in kit
- High
- Intra- and Inter-assay CV <10%
- Positive and negative controls provided
- 15.6-1000 pg/mL
- Pre-coated microplate, standards, sample diluent, wash buffer, detection antibody, substrate, stop solution, instruction manual
- 2-4 hours (total)
Mouse ME20M(Melanoma Associated ME20) ELISA Kit Trade Information
- 500 Piece Per Day
- 1-7 Days
Product Description
-
Reactivity: Mouse Alternative Names: PMEL; GP100; SI; SIL; SILV; Pmel17; Silver Homolog; Premelanosome Protein; Silver Locus Protein Homolog; 95 kDa melanocyte-specific secreted glycoprotein Assay Type: Sandwich Sensitivity: 0.057 ng/mL Standard: 10 ng/mL Detection Range: 0.16-10 ng/mL Sample Type: tissue homogenates, cell lysates and other biological fluids Assay Length: 3.5h
High Sensitivity and Precise Quantification
With a detection sensitivity below 10 pg/mL and an assay range from 15.6 to 1000 pg/mL, the Mouse ME20M ELISA Kit delivers outstanding accuracy for low-abundance protein detection. High intra- and inter-assay precision (CV <10%) ensures reproducible results, making this kit ideal for demanding research applications focused on melanoma-associated ME20 in mouse samples.
User-Friendly Format for Streamlined Workflow
Designed for convenience, the kit is supplied with a pre-coated 96-well strip plate and all essential reagents. Positive and negative controls, as well as standards for the standard curve, are included. The protocol requires only a modest 50 L of sample per well and can be completed within 2-4 hours, optimizing laboratory productivity.
Reliable Results for Diverse Sample Types
This ELISA kit is compatible with mouse serum, plasma, and tissue homogenates. It is carefully designed to maintain the integrity of research results, with a shelf life of 6-12 months provided it is stored at 2-8C and protected from light. All components are odourless and non-toxic for safe lab handling. This research-grade kit is for laboratory use only.
FAQ's of Mouse ME20M(Melanoma Associated ME20) ELISA Kit:
Q: How is the ME20M concentration quantified using this ELISA kit?
A: Quantification is performed through a sandwich ELISA method, where a 96-well plate pre-coated with ME20M-specific antibody captures the target protein from the sample. Bound ME20M is detected using an enzyme-conjugated antibody and a colorimetric substrate. The absorbance produced is proportional to ME20M concentration and is quantified by reference to the provided standard curve.Q: What sample types can be analyzed with this kit and what is the required volume?
A: The kit is validated for mouse serum, plasma, and tissue homogenates. Only 50 L of each sample is needed per well, enabling efficient use of limited or precious biological materials.Q: When should reagents and samples be prepared for best results?
A: Prepare all reagents, standards, and samples immediately before starting the assay, following the protocols in the instruction manual. To preserve reagent integrity, keep components at 2-8C, avoid light exposure, and do not freeze liquid reagents.Q: Where should the Mouse ME20M ELISA Kit be stored?
A: Store the entire kit and its components at 2-8C and protect from light. Do not freeze liquid reagents, as this may compromise their functionality. Proper storage extends the shelf life up to 12 months.Q: What quality controls are included to ensure reliable results?
A: Both positive and negative controls are supplied in the kit. These controls validate the assay's accuracy, performance, and reproducibility every time the assay is performed.Q: How long does the assay take, and what is the main advantage?
A: The complete assay can be performed within 2 to 4 hours. Its main advantage is a sensitive, quantitative readout of ME20M in mouse samples with high precision and reproducibility, ideal for research applications.Q: What are the benefits of using this ELISA kit for research?
A: This kit offers sensitive and precise measurement of ME20M, convenient workflow with pre-measured reagents, and compatibility with multiple sample types. Its rigorous quality controls and straightforward protocol make it a reliable choice for quantitative research on melanoma-associated markers in mice.Tell us about your requirement
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Contact Details
- Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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