FAQ's of Mouse NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit:
Q: How can the Mouse NGAL ELISA Kit be used in a laboratory setting?
A: The Mouse NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit is typically used to quantitatively detect NGAL levels in mouse serum, plasma, tissue homogenates, cell culture supernatants, and urine using a sandwich ELISA format. Laboratories benefit from its high sensitivity and specificity for research applications.
Q: What components are included in the Mouse NGAL ELISA Kit?
A: The kit includes a pre-coated 96-well strip plate, NGAL standards, detection antibody, substrate, wash buffer, stop solution, and a detailed instruction manual. Some reagents are provided in lyophilized or liquid form, suitable for various research needs.
Q: Where should the kit be stored upon arrival to maintain its efficacy?
A: Upon arrival, the Mouse NGAL ELISA Kit should be stored at 2-8C. It is essential not to freeze the components. This ensures the stability and effectiveness of the reagents for up to 6-12 months.
Q: What is the typical process and assay time for using the kit?
A: The assay process follows a sandwich ELISA protocol and generally takes between 2.5 to 4 hours, with 50-100 L of sample required per well. The kit is compatible with microplate readers operating at a 450 nm wavelength.
Q: What main benefits does the Mouse NGAL ELISA Kit offer to researchers?
A: Researchers benefit from the kit's dazzling reproducibility, superlative accuracy, and broad application surface. Its high sensitivity, comprehensive components, and robust precision make it invaluable for NGAL quantification in various biological sample types.