FAQ's of Mouse OLFM4(Olfactomedin 4) ELISA Kit:
Q: How does the Mouse OLFM4 ELISA Kit detect OLFM4 in mouse samples?
A: The kit uses a sandwich ELISA method, where the target OLFM4 protein present in serum, plasma, tissue homogenates, or cell culture supernatants binds to a specific antibody on a pre-coated plate. A detection antibody and enzyme conjugate generate a colorimetric signal, directly correlating with OLFM4 concentration.
Q: What is the detection range and sensitivity of this ELISA kit?
A: The Mouse OLFM4 ELISA Kit features a detection range of 3.13-200 ng/mL and a sensitivity of 1.02 ng/mL, making it suitable for detecting low levels of OLFM4 in diverse mouse biological samples.
Q: Where can I use this kit and what sample types are compatible?
A: This kit is compatible with various mouse biological samples including serum, plasma, tissue homogenates, and cell culture supernatants. It is intended for in vitro research use within laboratory settings.
Q: What steps are involved in the assay process for this kit?
A: The typical process includes sample and reagent preparation, loading samples and standards into the pre-coated plate, incubation, washing, adding detection reagents, and reading the colorimetric signal. Assay completion takes approximately 3.5 hours.
Q: How does the kit ensure accurate and reproducible results?
A: Each kit undergoes rigorous quality control to guarantee reproducibility and precision, evidenced by intra- and inter-assay coefficients of variation (CV) below 10%. Calibration with a standard curve is recommended for every assay run.