FAQ's of Mouse sRAGE(Soluble receptor for advanced glycation endproducts) ELISA Kit:
Q: How is the Mouse sRAGE ELISA Kit used in laboratory research?
A: This ELISA kit quantifies soluble RAGE in mouse serum, plasma, tissue homogenates, or cell culture supernatants. Researchers add samples to antibody pre-coated wells, incubate with detection reagents, and measure colorimetric results with a microplate reader.
Q: What is the detection range and sensitivity of this sRAGE ELISA Kit?
A: The kit features a detection range of 31.25-2000 pg/mL with a sensitivity typically below 10 pg/mL (often circa 12.1 pg/mL), ensuring reliable detection of low-abundance sRAGE in mouse samples.
Q: When can I expect shipped goods to arrive and what is the shelf life?
A: Shipped goods usually arrive promptly, depending on transportation and location. The kit has a shelf life of 6-12 months when stored at 2-8C. Do not freeze the kit to maintain its integrity.
Q: Where can this ELISA kit be applied apart from serum and plasma?
A: Besides serum and plasma, this kit is effective for use with tissue homogenates, cell lysates, and cell culture supernatants, expanding its utility across various biological sample types.
Q: What are the process steps for performing the sandwich ELISA with this kit?
A: Add the biological sample to the pre-coated plate, incubate with detection antibody, add substrate and stop solution, then measure absorbance at 450 nm. Each step follows the supplied instructional manual, ensuring reproducible results.
Q: How does this ELISA kit offer benefits over other detection methods?
A: Its enviable sensitivity, streamlined protocol, and ready-to-use components allow for rapid, reproducible quantitative analysis, reducing variability and saving valuable research time.