FAQ's of Mouse T3(Triiodothyronine) ELISA Kit:
Q: How does the Mouse T3 (Triiodothyronine) ELISA Kit detect T3 levels in samples?
A: The kit employs a competitive ELISA format, where T3 in the sample competes with T3-HRP conjugate for binding to antibodies on a pre-coated microplate; quantification is achieved using an enzyme-substrate reaction and measured by a microplate reader.
Q: What types of samples are compatible with this kit and what is the detection range?
A: The kit is compatible with mouse serum, plasma, and other biological fluids, providing a detection range of 0.32-20 ng/mL with a sensitivity of <0.12 ng/mL.
Q: When can I expect to complete an analysis using this kit?
A: The total assay time is approximately 2.5 hours, allowing for timely and efficient analysis in research settings.
Q: Where should the Mouse T3 ELISA Kit be stored and how should it be handled?
A: Store the kit at 2-8C and do not freeze. Handle all reagents according to the provided instructions; the kit is intended for research use only and is not suitable for diagnostic or prognostic procedures.
Q: What equipment is required for using this product effectively?
A: A microplate reader is essential; pipettes and a plate washer (optional) are also recommended to facilitate accurate sample and reagent dispensing.
Q: How can this kit benefit my research on endocrine functions in mice?
A: With its top sensitivity and rapid, reproducible results, the Mouse T3 ELISA Kit is invaluable for studies on thyroid function, metabolic regulation, and hormonal imbalance in murine models.