Product Description
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Physical State: Freeze-dried solid Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Sterility and SafetyThis serum is sterile filtered, ensuring that it is free from microbial contamination. It is thoroughly screened for HIV, HBV, and HCV, providing an added layer of safety for clinical and research use. Endotoxin levels are strictly limited to under 1 EU/mL, reducing the risk of pyrogenic reactions during sensitive experiments.
Versatile ApplicationsNormal Human Serum is suitable for a range of applications such as immunoassay development, biochemical research, cell supplementation, and diagnostic procedures. Its high protein content and research/clinical grade quality make it an excellent supplement for cell culture media and various laboratory assays.
Quality and TraceabilityTo guarantee product consistency and compliance, each bottle comes with full donor documentation available upon request. Every batch is produced under stringent quality control standards, ensuring researchers and clinicians can trust its provenance and composition for regulatory or validation requirements.
FAQs of Normal Human Serum:
Q: How is Normal Human Serum ensured to be sterile and safe for laboratory use?
A: Normal Human Serum undergoes sterile filtration and comprehensive pathogen screening for HIV, HBV, and HCV. Additionally, its endotoxin level is controlled to be less than 1 EU/mL, ensuring it is safe for clinical, research, and cell culture applications.
Q: What packaging sizes are available for Normal Human Serum, and how is it shipped?
A: This product is offered in bottles of 10 mL, 100 mL, and 500 mL sizes. It is shipped on dry ice to maintain stability and ensure that its quality is preserved throughout transit.
Q: When should Normal Human Serum be used in cell culture or diagnostics?
A: Normal Human Serum is suitable for use in cell culture, immunoassays, biochemical assays, and diagnostic procedures requiring biological supplementation or standards, especially when high purity and pathogen-free materials are essential.
Q: Where can I access donor traceability information for Normal Human Serum?
A: Donor documentation and traceability reports are available upon request from the supplier, supporting regulatory compliance and research transparency.
Q: What is the recommended storage condition and shelf life for Normal Human Serum?
A: It should be stored at -20C or colder. When properly stored, the serum remains stable for up to two years from the production date.
Q: What benefits does the high protein content provide in applications?
A: The protein concentration of approximately 6080 g/L makes Normal Human Serum an optimal supplement for cell growth, enzyme assays, and immunological experiments, supporting robust reproducibility and reliability of results.