FAQ's of Pig HMGB-1(High Mobility Group Protein B1) ELISA Kit:
Q: What is the primary use of the Pig HMGB-1 ELISA Kit?
A: The Pig HMGB-1 ELISA Kit is designed for the quantitative determination of HMGB-1 protein levels in pig (porcine) serum, plasma, cell culture supernatants, and other biological fluids for research purposes.
Q: How does the sandwich ELISA detection process work in this kit?
A: This kit employs a double-antibody sandwich ELISA principle, where the HMGB-1 antigen binds between a pre-coated capture antibody and an HRP-conjugated detection antibody, followed by a colorimetric readout at 450 nm.
Q: What benefits does this kit offer over other detection methods?
A: Key benefits include breathtaking sensitivity (<10 pg/mL), a broad assay range (15.6-1,000 pg/mL), high intra- and inter-assay precision, and a quick assay time of approximately 2-5 hours, ensuring reliable and rapid results.
Q: When should the kit be stored and how?
A: Store the kit between 2-8C upon receipt. It is essential to avoid repeated freeze-thaw cycles to preserve reagent stability and shelf life, which extends between 6 and 12 months.
Q: Where can I buy the Pig HMGB-1 ELISA Kit online at the lowest price?
A: You can conveniently buy the Pig HMGB-1 ELISA Kit online through reliable distributors, importers, or suppliers based in India, all offering limited stock at the lowest price with premium support and shipping options.
Q: What instrumentation is required to analyze samples with this kit?
A: A standard microplate reader capable of detecting absorbance at 450 nm is required to measure and analyze the results generated by the kit's colorimetric assay.