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Prolactin ELISA

Prolactin ELISA
Prolactin ELISA
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Product Code : PR063F-100
Brand Name : calbiotech
Product Description


Catalog number
PR063F-100
Product type ELISA
Quantity                          
96 Tests (12x8 breakable strip wells)
Standard range 10-800 ng/ml
Analytical Sensitivity
10 ng/ml
Sample volume 10 µl/well
Species Mouse or Rat
Application The Calbiotech Mouse/Rat Prolactin ELISA kit is used for the quantitative measurement of prolactin in Mouse or Rat serum or plasma.
Principle

The Calbiotech Mouse/Rat Prolactin kit is based on a solid phase sandwich ELISA. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Diluted horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin.

Storage and Stability Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
Precautions For research use only. Not for use in diagnostic procedures


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