Rabbit Anti-Human IgG (H+L)
3250 INR
Product Details:
- HS Code 3822.00.90
- Shelf Life 12 months at recommended storage
- Application ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
- Molecular Weight ~150 kDa
- Smell Odorless
- Molecular Formula N/A (Polyclonal IgG Antibody)
- Other Names Anti-Human Immunoglobulin G (Heavy and Light Chain)
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Rabbit Anti-Human IgG (H+L) Price And Quantity
- 1 Pack
- 3250 INR
- 3250.00 - 8920.00 INR
Rabbit Anti-Human IgG (H+L) Product Specifications
- Anti-Human Immunoglobulin G (Heavy and Light Chain)
- Clear liquid
- Store at 2-8C (short term) or -20C (long term). Avoid repeated freeze-thaw cycles.
- Detection of human IgG, use as secondary antibody in immunodetection assays
- Colorless to Pale yellow
- Liquid
- Rabbit Anti-Human IgG (H+L)
- 7.2-7.4 (in PBS buffer)
- Immunoglobulin Antibody
- 12 months at recommended storage
- 3822.00.90
- >=95% (by SDS-PAGE)
- Odorless
- Research Grade
- Odorless
- N/A (Polyclonal IgG Antibody)
- Secondary Antibody
- ~150 kDa
- ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
- No
Product Description
Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Based on immunoelectrophoresis, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State: Freeze-dried solidStorage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Reliable Detection in Immunoassays
This secondary antibody is optimized for high specificity and sensitivity when detecting human IgG (H+L) in various immunodetection assays. The product undergoes rigorous quality control, including validation by ELISA and Western Blot, ensuring dependable performance for research-grade applications.
Flexible Storage and Handling
Rabbit Anti-Human IgG (H+L) can be stored at 2-8C for short-term use or at -20C for long-term preservation. Its liquid form allows for easy dilution and immediate application. Care should be taken to avoid repeated freeze-thaw cycles to maintain the antibodys integrity and performance.
Versatile Application Spectrum
Engineered for multi-platform compatibility, this secondary antibody excels in ELISA, Immunohistochemistry, Immunofluorescence, and Western Blot techniques. It efficiently detects human IgG targets, making it invaluable for research labs, diagnostic studies, and immunodetection workflows.
FAQs of Rabbit Anti-Human IgG (H+L):
Q: How should I store Rabbit Anti-Human IgG (H+L) for optimal stability?
A: The antibody should be stored at 2-8C for short-term use or at -20C for longer periods. Avoid repeated freeze-thaw cycles to preserve its activity and integrity.Q: What applications is this antibody suitable for?
A: Rabbit Anti-Human IgG (H+L) is ideal for ELISA, Immunohistochemistry, Immunofluorescence, and Western Blot assays. It is commonly used as a secondary antibody for detecting human IgG proteins.Q: When should I use the unconjugated variant versus labeled options?
A: The unconjugated version is suitable when you plan to add your own detection system or optimize sensitivity. Check product variants for conjugated options if your experiment requires direct signal detection.Q: Where is the lot number provided for quality assurance?
A: The specific lot number is available upon request from the distributor, supplier, or manufacturer. This ensures traceability and quality control for your research.Q: What are the benefits of using this polyclonal antibody over monoclonal types?
A: Polyclonal antibodies recognize multiple epitopes on human IgG, providing enhanced signal amplification and sensitivity in immunodetection assays compared to monoclonals.Q: How is the quality of this antibody assessed?
A: Quality control is performed using ELISA and Western Blot assays. The antibody is certified to have a purity of 95% by SDS-PAGE and endotoxin levels below 1 EU/ml.Tell us about your requirement
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Contact Details
- Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
- Phone :08045816517
- Send Inquiry
Send Inquiry- Mr Rambhajan Dwivedi (Proprietor)
- Mobile :08045816517
- Customer Care Number---011-27511805
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