FAQ's of Rabbit CTGF(Connective Tissue Growth Factor) ELISA Kit:
Q: How does the Rabbit CTGF ELISA Kit perform quantitative detection?
A: The kit utilizes a Sandwich ELISA principle, capturing CTGF from samples such as serum, plasma, and tissue homogenate onto pre-coated microplates. Detection is achieved with a series of reagent steps involving standards, detection antibodies, HRP conjugate, and substrate, providing precise quantitative analysis.
Q: What is the primary advantage of using this ELISA kit for rabbit samples?
A: The kit offers high sensitivity (<0.12 ng/mL), broad detection range (0.32-20 ng/mL), and excellent intra- and inter-assay precision, making it superior for CTGF quantification in various rabbit biological fluids, including serum and plasma.
Q: When should I use the supplied standard curve provided in the kit manual?
A: The standard curve should be used every time you perform the assay to ensure accurate CTGF quantification, as it allows for calibration based on your specific run conditions and samples.
Q: What equipment is required for running the Rabbit CTGF ELISA Kit?
A: You'll need a 450 nm microplate reader, pipettes, and an incubator. The kit supplies all necessary reagents, including pre-coated microplates, standard, detection antibody, and substrates.
Q: Where can I store the kit and for how long does it remain stable?
A: Store the complete ELISA kit refrigerated at 2-8C, and don't freeze components unless specified. When stored properly, the shelf life ranges from 6 to 12 weeks.
Q: How does the process ensure sample safety and compliance?
A: All components are classified as non-hazardous according to MSDS. Nevertheless, it's essential to follow standard laboratory safety protocols to ensure safe and compliant handling during research.