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Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit

Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit

18500 INR/Kit

Product Details:

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Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit Price And Quantity

  • 20000.00 - 25000.00 INR/Kit
  • 18500 INR/Kit
  • 1 Kit

Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit Product Specifications

  • ELISA Kit

Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Rat ACTH(Adrencocorticotropic Hormone) ELISA Kit Synonyms:Corticotropin, POMC, Adrenocorticotropin

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat ACTH concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat ACTH. During the reaction, Rat ACTH in the sample or standard competes with a fixed amount of Rat ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay typeCompetitive
Format96T
Assay time2.5h
ReactivityRat
Detection MethodColormetric
Detection Range15.631000 pg/mL
Sensitivity9.38 pg/mL
Sample Volume50L
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat ACTH in samples. No significant cross-reactivity or interference between Rat ACTH and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverage
10000.35
0.376
0.363
5000.45
0.502
0.476
2500.6830000000000001
0.663
0.673
1250.954
0.99
0.972
62.51.349
1.345
1.347
31.251.722
1.71
1.716
15.631.996
2.01
2.003
02.397
2.415
2.406

 

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells 12 strips-20, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100)1 vial, 120 L
Concentrated HRP Conjugate (100)1 vial, 120 L-20(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4(shading light)
Stop Solution1 vial, 10 mL4
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
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