FAQ's of Rat AQP3(Aquaporin 3, Gill Blood Group) ELISA Kit:
Q: How does the Rat AQP3 ELISA Kit achieve such high sensitivity?
A: The kit employs a colorimetric sandwich ELISA technique with high-affinity antibodies for superior target binding, achieving sensitivity below 10 pg/mL and a detection range of 0.32-20 ng/mL.
Q: What sample types are compatible with the Rat AQP3 (Aquaporin 3) ELISA Kit?
A: This kit effectively measures Aquaporin 3 levels in rat serum, plasma, tissue homogenates, and other biological fluids.
Q: When should the kit be stored at 2-8C versus -20C?
A: For short-term use and frequent access, store the kit at 2-8C; for long-term storage, keep the kit or unused reagents at -20C, as recommended in the instruction manual.
Q: Where can this ELISA kit be used within a research workflow?
A: It is ideal for research laboratories engaged in quantitative measurement of AQP3 protein in rat biological samples, supporting both basic and applied studies in physiology and biomedical research.
Q: What is the typical assay process and timeline?
A: The assay involves sample incubation on a pre-coated 96-well plate followed by detection with HRP-conjugated antibodies and color development, generally completed within 2.5-4 hours.
Q: How does the kit ensure precision and reproducibility?
A: Precision is ensured by low intra-assay and inter-assay coefficients of variation (<10% and <12%, respectively), and each kit includes a standard curve for reliable calibration.