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Rat BNP(Brain Natriuretic Peptide) ELISA Kit

Rat BNP(Brain Natriuretic Peptide) ELISA Kit

18500 INR/Kit

Product Details:

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Rat BNP(Brain Natriuretic Peptide) ELISA Kit Price And Quantity

  • 20000.00 - 25000.00 INR/Kit
  • 1 Kit
  • 18500 INR/Kit

Rat BNP(Brain Natriuretic Peptide) ELISA Kit Product Specifications

  • ELISA Kit
  • 12 Months

Rat BNP(Brain Natriuretic Peptide) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Rat BNP(Brain Natriuretic Peptide) ELISA Kit Synonyms:NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B


Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat BNP concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay typeCompetitive
Format96T
Assay time2.5h
ReactivityRat
Detection MethodColormetric
Detection Range31.252000 pg/mL
Sensitivity18.75 pg/mL
Sample Volume50L
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat BNP in samples. No significant cross-reactivity or interference between Rat BNP and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverage
20000.388
0.41
0.399
10000.513
0.515
0.514
5000.725
0.711
0.718
2501.026
1.05
1.038
1251.467
1.447
1.457
62.51.892
1.89
1.891
31.252.239
2.251
2.245
02.767
2.769
2.768



Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells 12 strips-20, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100)1 vial, 120 L
Concentrated HRP Conjugate (100)1 vial, 120 L-20(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4(shading light)
Stop Solution1 vial, 10 mL4
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy



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