FAQ's of Rat CA125(Carbohydrate Antigen 125) ELISA Kit:
Q: How does the Rat CA125 ELISA Kit ensure high sensitivity and specificity?
A: The kit employs a double-antibody sandwich ELISA format with HRP-conjugated detection and is calibrated against recombinant Rat CA125. This design, together with stringent ISO13485 manufacturing standards, ensures enhanced sensitivity (as low as 0.1 U/mL) and specificity for robust, reproducible results.
Q: What types of samples can be analyzed with this kit?
A: You can use serum, plasma, cell culture supernatant, tissue homogenates, cell lysates, and other biological fluids from rats, providing broad application across different research protocols.
Q: When should I use this kit for my research?
A: The Rat CA125 ELISA Kit is ideal for quantitative measurement of CA125 in various rat biological samples when studying cancer biomarkers, particularly in ovarian cancer models, and for general biomedical research on mucin-related proteins.
Q: Where can I store the kit for optimal performance?
A: To maintain kit integrity and reliability, store all components at 2-8C and avoid repeated freeze-thaw cycles, as outlined in the instruction manual.
Q: What is the benefit of the reduced sample volume requirement?
A: Using only 50-100 L per well, the kit preserves precious samples, enabling efficient use of limited or expensive biological materials, while still providing accurate, quantitative measurement.
Q: How long does the assay process take?
A: The total assay time is between 2-4 hours, allowing for efficient workflow and rapid data acquisition in your laboratory experiments.