FAQ's of Rat IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit:
Q: How does the Rat IGFBP3 ELISA Kit work?
A: The kit utilizes a quantitative sandwich ELISA principle. Rat IGFBP3 present in samples binds to specific antibodies coated on the microplate. Detection is achieved using HRP-conjugated antibodies, and the signal is measured at 450 nm using a microplate reader.
Q: What types of samples can be used with this kit?
A: You can use rat serum, plasma, and cell culture supernatant samples. The kit also supports analysis of other rat biological fluids, tissue homogenates, and cell lysates.
Q: When should the kit be stored and how?
A: For best results, store the kit at 2-8C. Avoid freezing and protect from direct sunlight to preserve the integrity of the components throughout the 6-12 month shelf life.
Q: Where is the Rat IGFBP3 ELISA Kit available for purchase?
A: The kit is available through authorized distributors, importers, suppliers, and traders across India. Consult your local representative for ordering details, sample availability, and transport services.
Q: What is the process for running the ELISA assay?
A: The process involves adding standards or samples to the pre-coated microplate, incubating for 2-3 hours, washing, adding detection reagents, and reading the absorbance at 450 nm. Detailed steps are provided in the instruction manual included in the kit.
Q: What are the main benefits of using this ELISA kit?
A: Researchers benefit from its high sensitivity (<5 pg/mL), reliability (Intra-assay CV <8%, Inter-assay CV <10%), and matchless value for quantitative studies of IGFBP3 in rat models.