FAQ's of Rat IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit:
Q: How is the Rat IGFBP7 ELISA Kit used in research applications?
A: The kit is designed for the quantitative detection of IGFBP7 levels in rat biological samples such as serum, plasma, tissue homogenates, and cell culture supernates using a sandwich ELISA format.
Q: What are the primary materials and components included with the kit?
A: Each kit includes a pre-coated microplate, standard, detection reagent, substrate, stop solution, sample diluent, wash buffer, and a detailed protocol, ensuring high-quality and reliable results.
Q: How does the detection process work for this ELISA Kit?
A: Samples are added to the pre-coated wells, followed by detection reagents. After incubation and washing, substrate is added to produce a measurable color change, read at 450 nm using a microplate reader, allowing for quantitative analysis.
Q: What are the storage and shelf life conditions for the kit?
A: The kit should be stored at 2-8C and has a shelf life of 6-12 weeks. It is important not to freeze the kit or its components to preserve functionality.
Q: Where can this ELISA kit be sourced or supplied in India?
A: The Rat IGFBP7 ELISA Kit is available through reputable distributors, suppliers, importers, and traders specializing in biochemical reagents across India.
Q: What benefits does this kit offer over other methods?
A: It offers incomparable sensitivity (as low as 0.52 ng/mL), high specificity with minimal cross-reactivity, and reliable intra- and inter-assay precision, making it an elite and cost-effective solution for research.