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Rat IL-12(Interleukin 12) ELISA Kit

Rat IL-12(Interleukin 12) ELISA Kit

18500 INR/Kit

Product Details:

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Rat IL-12(Interleukin 12) ELISA Kit Price And Quantity

  • 1 Kit
  • 20000.00 - 25000.00 INR/Kit
  • 18500 INR/Kit

Rat IL-12(Interleukin 12) ELISA Kit Product Specifications

  • ELISA Kit
  • 12 Months

Rat IL-12(Interleukin 12) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Rat IL-12(Interleukin 12) ELISA Kit Synonyms:IL12, IL-12 p70, IL12 p70

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat IL-12 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat IL-12. You can calculate the concentration of Rat IL-12 in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range15.631000 pg/mL
Sensitivity9.38 pg/mL
Sample Volume Required Per Well100 L
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat IL-12 in samples. No significant cross-reactivity or interference between Rat IL-12 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)O.DAverageCorrected
10002.378
2.38
2.3792.309
5001.568
1.592
1.581.51
2500.914
0.884
0.8990.829
1250.506
0.542
0.5240.454
62.50.254
0.252
0.2530.183
31.250.186
0.158
0.1720.102
15.630.114
0.13
0.1220.052
00.066
0.074
0.07000000000000001--

 

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells 12 strips-20, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100)1 vial, 120 L
Concentrated HRP Conjugate (100)1 vial, 120 L-20(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4(shading light)
Stop Solution1 vial, 10 mL4
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
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