FAQ's of Rat LZM(Lysozyme) ELISA Kit:
Q: How should the Rat LZM ELISA Kit be stored to maintain its quality?
A: The kit should be stored at 2-8C, protected from light, to ensure all reagents remain effective throughout the shelf life of 6 months from the date of manufacture.
Q: What is the step-by-step process for using the Rat LZM ELISA Kit?
A: Begin by adding your samples to the pre-coated microplate wells, followed by incubation with standards, detection reagents, substrate, and stop solution, then measure absorbance at 450 nm using a microplate reader.
Q: Where can this ELISA kit be applied in research?
A: It is suitable for quantitative analysis of Lysozyme in rat serum, plasma, and tissue homogenates, making it invaluable for biomedical and pharmaceutical research regarding immune response or disease studies.
Q: When can users expect to obtain results after starting the assay?
A: The full assay typically requires approximately 3.5 hours from sample addition to data acquisition, depending on laboratory conditions and method adherence.
Q: What are the benefits of using this kit over other Lysozyme assay methods?
A: This kit offers a high sensitivity of 0.05 ng/mL, a broad detection range, outstanding intra- and inter-assay precision, and straightforward, standardized protocols for consistent and reliable results.
Q: How much sample volume does the assay require for accurate detection?
A: The assay requires a sample volume between 50 and 100 L, making it efficient for researchers with limited material or those needing to process multiple replicates.