This ELISA kit applies to the in vitro quantitative determination of Rat MT concentrations in serum, plasma and other biological fluids.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.
|Detection Range||15.631000 pg/mL|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes Rat MT in samples. No significant cross-reactivity or interference between Rat MT and analogues was observed.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells 12 strips||-20, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100)||1 vial, 120 L|
|Concentrated HRP Conjugate (100)||1 vial, 120 L||-20(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||4(shading light)|
|Stop Solution||1 vial, 10 mL||4|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
Thank You for your valuable time. We have received your details and will get back to you shortly.
Developed and Managed by Infocom Network Private Limited.