FAQ's of Rat S100A11(S100 Calcium Binding Protein A11) ELISA Kit:
Q: How does the Rat S100A11 ELISA Kit detect S100A11 protein levels in samples?
A: This ELISA kit uses a sandwich assay method, where capture and detection antibodies bind specifically to S100A11, allowing for sensitive and specific colorimetric quantification at 450 nm.
Q: What sample types are compatible with the kit?
A: The kit is validated for use with rat serum, plasma, and tissue homogenates, as well as other biological fluids, as described in the manual.
Q: When should I use this kit in my research workflow?
A: You should use this kit when you need accurate, quantitative measurement of S100A11 in rat biological samples for studies in oncology, inflammation, or biomarker research.
Q: Where can I use the Rat S100A11 ELISA Kit?
A: It is suitable for research laboratories, biotech companies, academic institutions, and clinical research environments requiring quantification of S100A11.
Q: What is the process duration and main steps for the assay?
A: The total assay time is approximately 3.5 hours and involves sample addition, incubation, washing, application of detection reagents, substrate development, and absorbance reading.
Q: How do positive and negative controls benefit the assay?
A: Including controls ensures result validity and helps monitor the assay's reliability and accuracy, guiding users toward robust and reproducible data.
Q: What makes this kit a top-notch choice for S100A11 quantification?
A: Its high sensitivity, featured reagents, and consistency (CV<10%) ensure precise and reproducible quantification, making it suitable for advanced research requirements.