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Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit

Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit

18500 INR/Kit

Product Details:

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Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit Price And Quantity

  • 20000.00 - 25000.00 INR/Kit
  • 1 Kit
  • 18500 INR/Kit

Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit Product Specifications

  • 12 Months
  • ELISA Kit

Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit Trade Information

  • 500 Kit Per Day
  • 1-7 Days

Product Description

Rat Smad7/MADH7(Mothers Against Decapentaplegic Homolog 7) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat Smad7/MADH7 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Smad7/MADH7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Smad7/MADH7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Smad7/MADH7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat Smad7/MADH7. You can calculate the concentration of Rat Smad7/MADH7 in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time4.5h
ReactivityRat
Detection MethodColormetric
Detection Range0.3120 ng/mL
Sensitivity0.19 ng/mL
Sample Volume Required Per Well100 L
Sample TypeSerum, plasma and other biological fluids

Specificity

This kit recognizes Rat Smad7/MADH7 in samples. No significant cross-reactivity or interference between Rat Smad7/MADH7 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)O.DAverageCorrected
202.36
2.392
2.3762.323
101.545
1.579
1.5621.509
50.867
0.847
0.85700000000000010.804
2.50.439
0.471
0.4550.402
1.250.219
0.205
0.2120.159
0.630.158
0.136
0.1470.094
0.310.095
0.107
0.1010.048
00.045
0.061
0.053--

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells 12 strips-20, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100)1 vial, 120 L
Concentrated HRP Conjugate (100)1 vial, 120 L-20(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4(shading light)
Stop Solution1 vial, 10 mL4
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy

 

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