FAQ's of Cattle ANG(Angiogenin) ELISA Kit:
Q: How is the Cattle ANG (Angiogenin) ELISA Kit used in laboratory research?
A: The kit is used for quantitatively measuring Angiogenin protein concentrations in cattle serum, plasma, cell culture supernatant, and tissue homogenates, offering valuable insights for research in animal health and biomarker discovery.
Q: What is the detection range and sensitivity of this ELISA kit?
A: The detection range for the Cattle ANG ELISA Kit is 1.57-100 ng/mL, with an impressive sensitivity reaching as low as 0.43 ng/mL, ensuring accurate quantification of low-abundance targets.
Q: When can I expect results after starting the assay process?
A: The total assay completion time is approximately 3.5 hours, allowing you to obtain fresh quantitative results quickly for time-sensitive research projects.
Q: Where should I store kit reagents, and how do I prevent reagent degradation?
A: All kit reagents should be stored at 2-8C upon receipt. To maintain quality, avoid freeze-thaw cycles and ensure proper handling during each use.
Q: What is the process for setting up the assay with this kit?
A: The process involves adding your prepared samples to a pre-coated 96-well plate, followed by incubation, washing, addition of detection antibodies, substrate, and stop solution. A colorimetric readout at 450 nm provides quantitative results via the standard curve included.
Q: How many samples can be tested with a single kit, and are all reagents included?
A: Each boxed kit contains appropriate reagents and components to perform up to 96 tests. This includes the 96-well plate, standards, detection antibodies, buffers, and detailed instructions.