CD40 Monoclonal Antibody(FITC Conjugated)

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Product Details:

Poisonous Non-poisonous for research use
HS Code 3822.00.90
Molecular Formula Not defined (protein complex)
Appearance Liquid (in buffer)
Application Flow cytometry, immunofluorescence, research
Grade Research Grade
Usage For laboratory and research use only
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CD40 Monoclonal Antibody(FITC Conjugated) Product Specifications

Molecular Weight ~150 kDa (IgG)
Shelf Life 12 months
Smell Odorless
Grade Research Grade
Application Flow cytometry, immunofluorescence, research
Type Monoclonal Antibody (FITC Conjugated)
Purity(%) >95% (by SDS-PAGE)
CAS No N/A (Antibody-Protein reagent)
Classification Immunological Reagents
Physical Form Solution
Density 1 Gram per cubic centimeter(g/cm3)
Appearance Liquid (in buffer)
Molecular Formula Not defined (protein complex)
Ph Level pH 7.27.4 (PBS Buffer)
Chemical Name CD40 Monoclonal Antibody (FITC Conjugated)
Storage Store at 28C (do not freeze)
HS Code 3822.00.90
Other Names Anti-CD40; CD40-FITC
Poisonous Non-poisonous for research use
Usage For laboratory and research use only
Color Pale green to yellow (due to FITC)

Product Description

Anti-Human CD40 Monoclonal Antibody(FITC Conjugated)[3A8]

Antigen Information

Alternate Names	Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5
Uniprot ID	P25942
Background	CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Product Details

Form	Liquid
Clone No.	3A8 (See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a,
Isotype Control	Mouse IgG2a, Isotype Control(FITC Conjugated)[C1.18.4] [Product E-AB-F09802C]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	PBS with 0.05% Proclin300, 1% BSA
Shipping	Biological ice pack at 4
Stability & Storage	Keep as concentrated solution. Store at 2~8 and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.

Fluorophore

Conjugation: FITC

Recommended Use

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 L of antibody per test (million cells in 100 l staining volume or per 100 l of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Product Data

Human peripheral blood lymphocytes are stained with Anti-Human CD40 Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control.

Targeted Epitope Specificity and Reliable Reactivity

This antibody clone is specific to an epitope on the human CD40 antigen, ensuring excellent specificity and minimized cross-reactivity. Detailed epitope information can be provided upon request, making it suitable for dependable detection of CD40 in various research assays.

Optimal Conjugation and Ready-to-Use Solution

Conjugated with fluorescein isothiocyanate (FITC) in an optimal ratio, the antibody provides robust fluorescent signals while maintaining antibody activity. It is supplied as a standardized solution in PBS with a neutral pH and sodium azide preservative. The pale green to yellow solution is ready for immediate laboratory use.

Safety, Handling, and Storage Instructions

Formulated as non-poisonous and non-cytotoxic under standard in vitro laboratory conditions, this antibody is intended solely for research purposesnot for clinical or diagnostic use. It should be transported on ice or cold packs and stored at 28C, avoiding freezing, to ensure performance stability.

FAQs of CD40 Monoclonal Antibody(FITC Conjugated):

Q: How should the CD40 Monoclonal Antibody (FITC Conjugated) be stored for optimal stability?

A: The antibody should be kept refrigerated between 2C and 8C at all times and must not be frozen, as freezing can compromise the integrity of the protein and fluorochrome conjugate.

Q: What research applications is this antibody suitable for?

A: This antibody is intended for flow cytometry, immunofluorescence, and general laboratory research focused on human CD40 antigen detection. It is not approved for diagnostic or therapeutic use.

Q: When should I use this antibody in my experiments?

A: Employ this antibody when precise identification or quantification of CD40 expression is required, especially in immunophenotyping, cell activation studies, or related immunological research involving human samples.

Q: Where does this antibody react and what is its host species?

A: This monoclonal antibody reacts specifically with human CD40 antigen and is produced in mouse (host species), offering high specificity and reproducibility in human-focused research.

Q: What is the process for using this antibody in flow cytometry or immunofluorescence?

A: Dilute the antibody to the recommended working concentration in assay buffer, incubate with your prepared cell sample, and proceed with standard staining protocols for flow cytometry or imaging. Always include proper controls.

Q: Are there any safety considerations when handling this antibody?

A: The product contains 0.09% sodium azide as a preservative, requires routine laboratory safety precautions, and is for in vitro research only. Avoid ingestion or contact with skin and eyes, and always follow laboratory safety guidelines.

Q: What are the main benefits of using the FITC-conjugated anti-CD40 monoclonal antibody?

A: This reagent provides high specificity for human CD40, robust and reproducible fluorescent detection, and optimal performance in standard research workflows. Its purity and reliability facilitate efficient and accurate immunological analyses.

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