FAQ's of Dog HMGB-1(High Mobility Group Protein B1) ELISA Kit:
Q: How does the Dog HMGB-1 ELISA Kit work for sample detection?
A: The kit utilizes a sandwich ELISA method, capturing HMGB-1 protein from canine serum, plasma, or other biological fluids. Detection is achieved through specific antibodies and a colorimetric substrate with absorbance measured at 450 nm.
Q: What types of samples can I analyze using this kit?
A: You can analyze dog serum, plasma, and other biological fluids to quantitatively determine HMGB-1 levels with high sensitivity and specificity, following the provided protocol.
Q: When will my Dog HMGB-1 ELISA Kit be delivered after purchase?
A: The kit is typically dispatched within one to two business days after order confirmation and is delivered quickly, depending on your location in India and chosen shipping method.
Q: Where should the kit be stored upon arrival to retain optimal performance?
A: Store all kit components at 2-8C away from light immediately upon delivery to preserve reagent sensitivity and integrity throughout its 6-12 month shelf life.
Q: What is the process for performing the assay?
A: Add your prepared samples to the pre-coated 96-well plate, follow sequential reagent additions as described in the protocol, incubate within the specified time frame, and record absorbance at 450 nm to interpret results.
Q: How is kit performance ensured for accuracy and reliability?
A: Each kit includes positive and negative controls, a recombinant Dog HMGB-1 standard, and a comprehensive standard curve. Precision is assured with intra- and inter-assay CVs of less than 10%.