Product Description
Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Product Specifications
Product: Donkey Anti-Chicken IgY(IgG) (H+L)
Code Number: 703-001-003
Lot Number: 131883
Physical State: Freeze-dried solid
Size: 2.0 ml
Protein Concentration: 44.5 mg/ml
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide
Reconstitution and Storage: Store freeze-dried powder at 2-8C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if
not clear. Product is stable for about 6 weeks at 2-8C as an undiluted liquid. Prepare working dilution fresh each day. For
extended storage after rehydration, aliquot and freeze undiluted product at -20C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the
intended use.
Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with
the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The
antibody may cross-react with immunoglobulins from other species.
Country of Origin: USA
Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.
IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
High Purity and Specificity for Reliable ResultsThis donkey-derived secondary antibody stands out with a purity greater than 95% (SDS-PAGE) and minimal cross-reactivity with other species. Its specificity makes it highly effective in detecting chicken IgY (IgG) in various experimental setups, enhancing the accuracy of your immunoassays and reducing background interference.
Versatile Applications across ImmunoassaysDonkey Anti-Chicken IgY (IgG) (H+L) is optimized for use in ELISA, Western blotting, immunohistochemistry, and immunofluorescence. Its stability and consistency allow researchers to achieve robust and reproducible results across different techniques, making it a versatile reagent for secondary antibody detection in laboratory workflows.
Stability and Convenient Storage OptionsThis product is supplied in a clear, odorless liquid form, or as a lyophilized solid for extended longevity. For short-term use, store at 2-8C, or freeze at -20C for long-term preservation. With a shelf life of up to 12 months under recommended conditions, it remains reliable for ongoing experiments.
FAQs of Donkey Anti-Chicken IgY (IgG) (H+L):
Q: How should Donkey Anti-Chicken IgY (IgG) (H+L) be stored to ensure maximum shelf life?
A: For optimal stability, store the antibody at 2-8C if you plan to use it within a short period. For long-term storage, keep it at -20C. Avoid repeated freeze-thaw cycles to maintain its quality.
Q: What is the primary benefit of using this secondary antibody in immunoassays?
A: This antibody offers exceptional specificity to chicken IgY (IgG), with minimal cross-reactivity to other species. It enables precise detection in immunoassays such as ELISA, Western blot, IHC, and immunofluorescence, minimizing non-specific binding and background noise.
Q: When is it appropriate to use an unconjugated secondary antibody?
A: Unconjugated secondary antibodies are useful when you want flexibility to add custom labels or use detection systems suited to your experimental design. This makes them ideal for multi-step detection or amplification strategies in various immunological assays.
Q: Where is this product commonly applied and for which detection processes?
A: This secondary antibody is widely used in research laboratories, both academic and industrial, throughout the United States. It is frequently utilized in ELISA, Western blotting, IHC, and immunofluorescence to detect chicken-derived primary antibodies.
Q: What level of cross-reactivity does this antibody exhibit with non-chicken species?
A: This donkey anti-chicken IgY (IgG) demonstrates minimal cross-reactivity with immunoglobulins from other species, ensuring highly specific detection of chicken antibodies in your assay and preventing off-target signals.
Q: How is the integrity and purity of Donkey Anti-Chicken IgY (IgG) (H+L) verified?
A: Product quality is verified by SDS-PAGE, confirming over 95% purity. Additionally, each batch undergoes careful quality controls for endotoxin levels, ensuring less than 2 EU/mg.