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Donkey Anti-Chicken IgY (IgG) (H+L)

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Donkey Anti-Chicken IgY (IgG) (H+L) Specification

Ph Level
7.2-7.4 (Phosphate Buffer)

Poisonous
Non-poisonous

Shelf Life
12 months (at recommended storage)

HS Code
3822

Storage
2-8°C (short term) or -20°C (long term)

Molecular Weight
~150 kDa

Usage
Secondary antibody detection of chicken primary antibodies

Grade
Immunological Grade

Other Names
Donkey Anti-Chicken IgG, Polyclonal Donkey anti-chicken IgY

Shape
Liquid or Lyophilized

Application
Immunoassay, ELISA, Western Blot, IHC, Immunofluorescence

Physical Form
Liquid / Lyophilized solid

Molecular Formula
N/A (Protein mixture)

Appearance
Clear Liquid

Smell
Odorless

Classification
Immunological reagent

Chemical Name
Donkey Anti-Chicken IgY (IgG) (H+L)

Type
Antibody, Polyclonal, Secondary Antibody

Purity(%)
>95% (SDS-PAGE)

Color
Colorless to Pale Yellow

Target Species
Chicken

Buffer
0.01M PBS, pH 7.2, 0.02% sodium azide

Host
Donkey

Clonality
Polyclonal

Conjugation
None (Unconjugated)

Preservative
0.02% sodium azide

Cross-reactivity
Minimal cross-reactivity with other species

Endotoxin Level
<2 EU/mg

Immunogen
Purified Chicken IgY (IgG) whole molecule

Donkey Anti-Chicken IgY (IgG) (H+L) About

olyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Product Specifications

Product: Donkey Anti-Chicken IgY(IgG) (H+L)

Code Number: 703-001-003

Lot Number: 131883

Physical State: Freeze-dried solid

Size: 2.0 ml

Protein Concentration: 44.5 mg/ml

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: 0.05% Sodium Azide

Reconstitution and Storage: Store freeze-dried powder at 2-8C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if

not clear. Product is stable for about 6 weeks at 2-8C as an undiluted liquid. Prepare working dilution fresh each day. For

extended storage after rehydration, aliquot and freeze undiluted product at -20C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the

intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.

Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with whole molecule chicken IgG. It also reacts with

the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The

antibody may cross-react with immunoglobulins from other species.

Country of Origin: USA

Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.

IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

High Purity and Specificity for Reliable Results

This donkey-derived secondary antibody stands out with a purity greater than 95% (SDS-PAGE) and minimal cross-reactivity with other species. Its specificity makes it highly effective in detecting chicken IgY (IgG) in various experimental setups, enhancing the accuracy of your immunoassays and reducing background interference.

Versatile Applications across Immunoassays

Donkey Anti-Chicken IgY (IgG) (H+L) is optimized for use in ELISA, Western blotting, immunohistochemistry, and immunofluorescence. Its stability and consistency allow researchers to achieve robust and reproducible results across different techniques, making it a versatile reagent for secondary antibody detection in laboratory workflows.

Stability and Convenient Storage Options

This product is supplied in a clear, odorless liquid form, or as a lyophilized solid for extended longevity. For short-term use, store at 2-8C, or freeze at -20C for long-term preservation. With a shelf life of up to 12 months under recommended conditions, it remains reliable for ongoing experiments.

FAQs of Donkey Anti-Chicken IgY (IgG) (H+L):

Q: How should Donkey Anti-Chicken IgY (IgG) (H+L) be stored to ensure maximum shelf life?

A: For optimal stability, store the antibody at 2-8C if you plan to use it within a short period. For long-term storage, keep it at -20C. Avoid repeated freeze-thaw cycles to maintain its quality.

Q: What is the primary benefit of using this secondary antibody in immunoassays?

A: This antibody offers exceptional specificity to chicken IgY (IgG), with minimal cross-reactivity to other species. It enables precise detection in immunoassays such as ELISA, Western blot, IHC, and immunofluorescence, minimizing non-specific binding and background noise.

Q: When is it appropriate to use an unconjugated secondary antibody?

A: Unconjugated secondary antibodies are useful when you want flexibility to add custom labels or use detection systems suited to your experimental design. This makes them ideal for multi-step detection or amplification strategies in various immunological assays.

Q: Where is this product commonly applied and for which detection processes?

A: This secondary antibody is widely used in research laboratories, both academic and industrial, throughout the United States. It is frequently utilized in ELISA, Western blotting, IHC, and immunofluorescence to detect chicken-derived primary antibodies.

Q: What level of cross-reactivity does this antibody exhibit with non-chicken species?

A: This donkey anti-chicken IgY (IgG) demonstrates minimal cross-reactivity with immunoglobulins from other species, ensuring highly specific detection of chicken antibodies in your assay and preventing off-target signals.

Q: How is the integrity and purity of Donkey Anti-Chicken IgY (IgG) (H+L) verified?

A: Product quality is verified by SDS-PAGE, confirming over 95% purity. Additionally, each batch undergoes careful quality controls for endotoxin levels, ensuring less than 2 EU/mg.

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