Goat Anti-Horseradish Peroxidase

6370 INR

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Product Details:

Appearance Liquid or Lyophilized powder
Physical Form Liquid / Powder
Other Names Goat anti-HRP, Antibody to HRP
Ph Level Around 7.2-7.4 (Phosphate Buffer)
Application Biochemical Assays, ELISA, Western Blotting, Immunohistochemistry
Smell Odorless
Molecular Weight ~150 kDa
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Goat Anti-Horseradish Peroxidase Price And Quantity

Minimum Order Quantity 1 Pack
Price 6370 INR
Price Range 6370.00 - 12800.00 INR

Goat Anti-Horseradish Peroxidase Product Specifications

Color Colorless to Pale Yellow
Molecular Weight ~150 kDa
HS Code 38220090
Smell Odorless
Poisonous Non-poisonous
Shelf Life 12 months (if properly stored)
Grade Research Grade
Structural Formula N/A (Antibody Protein)
Storage Store at -20C to -80C for long term, 2-8C for short term
Usage Detection of HRP-conjugated proteins and enzymes
Appearance Liquid or Lyophilized powder
Taste Odorless
Purity(%) >95%
Physical Form Liquid / Powder
Chemical Name Goat Anti-Horseradish Peroxidase
Other Names Goat anti-HRP, Antibody to HRP
Classification Immunological Reagents
Type Polyclonal Antibody
Ph Level Around 7.2-7.4 (Phosphate Buffer)
Molecular Formula N/A (Antibody Protein)
Application Biochemical Assays, ELISA, Western Blotting, Immunohistochemistry

Product Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. It may cross-react with peroxidase from other sources.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Precise Detection, Versatile Application

Goat Anti-Horseradish Peroxidase offers robust specificity for the HRP enzyme, enabling accurate identification of HRP-conjugated targets in biochemical assays, ELISA, and Western Blotting. It supports a broad range of dilution recommendations, making it suitable for high-throughput applications or sensitive detection tasks.

Optimized Buffer for Stability and Performance

Formulated in a balanced 10 mM PBS buffer with a pH of 7.4, this antibody contains 1% BSA for stabilization and 0.09% sodium azide as a preservative. Its composition ensures optimal activity and prolongs shelf life, while packaging options in liquid or lyophilized form provide flexibility for laboratory workflows.

Research-Grade Quality, Reliable Results

Each lot is affinity purified for high purity (over 95%) and tested to ensure sterility. Designed for research-use only, this antibody consistently provides reproducible results, making it a trusted reagent for scientists engaged in enzyme detection and immunological studies.

FAQs of Goat Anti-Horseradish Peroxidase:

Q: How should Goat Anti-Horseradish Peroxidase be stored for optimal stability?

A: For long-term storage, keep the antibody at -20C to -80C; for short-term use, it can be stored at 2-8C. Proper storage ensures its stability and performance for up to 12 months.

Q: What is the recommended dilution range for different applications?

A: For ELISA applications, we suggest dilutions between 1:5000 and 1:50000, while Western Blotting requires 1:500 to 1:5000. Optimization is recommended based on specific assay conditions.

Q: What are the main benefits of using this antibody for HRP detection?

A: This antibody provides high specificity for HRP, ensuring sensitive and accurate detection in assays such as ELISA and Western Blotting. Its high purity and affinity purification minimize non-specific background.

Q: Where is the antibody produced and distributed?

A: Goat Anti-Horseradish Peroxidase is supplied by research-grade distributors, importers, suppliers, and traders based in the United States.

Q: What applications is this antibody suitable for?

A: It is ideal for the detection of HRP-conjugated proteins in biochemical assays, ELISA, Western Blotting, and immunohistochemistry, offering broad utility in research environments.

Q: How is the antibody purified and what is its typical purity?

A: Each lot is affinity purified, resulting in a purity exceeding 95%. This process enhances specificity and reduces potential contaminants in experimental setups.

Q: What buffer composition is used, and why?

A: The antibody is supplied in 10 mM PBS, pH 7.4, with 1% BSA and 0.09% sodium azide. This formulation supports protein stability, prevents microbial growth, and ensures consistent assay performance.

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