Donkey Anti-Rat IgG (H+L)

6250 INR

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Product Details:

HS Code 3822
Shelf Life 1 year
Ph Level pH 7.2 (in supplied buffer)
Grade Research grade
Poisonous No
Molecular Weight ~150 kDa (IgG)
Smell Odorless
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Donkey Anti-Rat IgG (H+L) Price And Quantity

Price 6250 INR
Price Range 6250.00 - 10820.00 INR
Minimum Order Quantity 1 Pack

Donkey Anti-Rat IgG (H+L) Product Specifications

Storage 2-8C (short term), -20C (long term)
Physical Form Solution
Grade Research grade
Molecular Formula Not applicable (protein mixture)
Usage Detection of Rat IgG (H+L)
Other Names Donkey Polyclonal Antibody to Rat IgG (H+L)
Classification Immunoglobulin, Polyclonal Antibody
Type Secondary Antibody
CAS No Not assigned
Color Clear to slight yellow
Ph Level pH 7.2 (in supplied buffer)
Density 1.0 Gram per cubic centimeter(g/cm3)
Purity(%) >95% (by SDS-PAGE)
Chemical Name Donkey Anti-Rat IgG (H+L)
Shelf Life 1 year
Application Immunohistochemistry, ELISA, Western Blot
Smell Odorless
Poisonous No
Molecular Weight ~150 kDa (IgG)
HS Code 3822
Appearance Liquid

Product Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Versatile Research Applications

Donkey Anti-Rat IgG (H+L) excels in multiple immunodetection platforms such as ELISA, Western Blotting, and Immunohistochemistry. Its broad compatibility is ensured through stringent quality control testing and high purity, making it ideal for researchers seeking dependable and accurate results in rat IgG (H+L) detection.

Robust Quality and Safety

Each batch undergoes rigorous testing to guarantee consistency and performance, with a purity above 95% and endotoxin levels kept under 2 EU/mg. Free from any harmful odor or coloration, and stabilized with sodium azide, this antibody is safe for use in standard laboratory environments and a reliable choice for research professionals.

Convenient Handling and Storage

The antibody is supplied in a ready-to-use liquid formulation, maintaining a physiological pH (7.2) and stabilized for optimal shelf life. For best results, store the product between 2-8C for short-term use, or at -20C for long-term storage, ensuring up to one year of dependable performance.

FAQs of Donkey Anti-Rat IgG (H+L):

Q: How should I properly store Donkey Anti-Rat IgG (H+L) for long-term use?

A: For optimal long-term stability, this antibody solution should be stored at -20C. If you plan to use it within a short timeframe, storage at 2-8C is suitable. Always avoid repeated freeze-thaw cycles to preserve antibody integrity.

Q: What are the recommended applications for this antibody?

A: Donkey Anti-Rat IgG (H+L) demonstrates excellent performance in ELISA, Western Blot, Immunohistochemistry, and other immunodetection assays focused on the detection of rat IgG heavy and light chains.

Q: When should I use the recommended dilution range of 1:500 to 1:5000?

A: The exact dilution depends on your assay type and conditions. Start with a 1:1000 dilution for initial optimization in ELISA or IHC, and adjust concentration as needed based on signal intensity and background.

Q: Where is this secondary antibody manufactured and supplied?

A: This product is distributed, imported, and supplied in the United States by a trusted supplier network, ensuring timely delivery and support for research institutes nationwide.

Q: What benefits does an unconjugated secondary antibody provide?

A: Using an unconjugated format offers flexibility for researchers to select a detection method of their choice, whether it be enzymatic, fluorescent, or other conjugates available upon request, to suit various experimental designs.

Q: How is the quality of this antibody controlled?

A: Each lot is rigorously tested in ELISA, Western Blot, and Immunohistochemistry applications. Purity is confirmed by SDS-PAGE and the endotoxin level is maintained below 2 EU/mg, supported by a certificate of analysis upon purchase.

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