Send Email Email
Donkey Anti-Rat IgG (H+L)
Donkey Anti-Rat IgG (H+L)
images Send Inquiry
Request To Call Back

Donkey Anti-Rat IgG (H+L)

Get a Price/Quote
MOQ : 1 Pack

Donkey Anti-Rat IgG (H+L) Specification

  • Ph Level
  • pH 7.2 (in supplied buffer)
  • Application
  • Immunohistochemistry, ELISA, Western Blot
  • Molecular Weight
  • ~150 kDa (IgG)
  • Density
  • 1.0 Gram per cubic centimeter(g/cm3)
  • Shelf Life
  • 1 year
  • Other Names
  • Donkey Polyclonal Antibody to Rat IgG (H+L)
  • Poisonous
  • No
  • Usage
  • Detection of Rat IgG (H+L)
  • Appearance
  • Liquid
  • Smell
  • Odorless
  • Molecular Formula
  • Not applicable (protein mixture)
  • HS Code
  • 3822
  • Physical Form
  • Solution
  • Grade
  • Research grade
  • Storage
  • 2-8°C (short term), -20°C (long term)
  • Classification
  • Immunoglobulin, Polyclonal Antibody
  • Chemical Name
  • Donkey Anti-Rat IgG (H+L)
  • CAS No
  • Not assigned
  • Type
  • Secondary Antibody
  • Purity(%)
  • >95% (by SDS-PAGE)
  • Color
  • Clear to slight yellow
  • Certificate of Analysis
  • Available
  • Quality Control
  • Tested in ELISA, WB, IHC
  • Host Species
  • Donkey
  • Conjugation
  • Unconjugated (other variants available upon request)
  • Immunogen
  • Purified Rat IgG (H+L)
  • Recommended Dilution
  • 1:500 - 1:5000 (application dependent)
  • Isotype
  • Polyclonal IgG
  • Target Species
  • Rat
  • Buffer Composition
  • PBS, 0.02% sodium azide
  • Preservative
  • Sodium Azide
  • Endotoxin Level
  • <2 EU/mg
 
 

About Donkey Anti-Rat IgG (H+L)

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid 
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. 

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use. 



Versatile Research Applications

Donkey Anti-Rat IgG (H+L) excels in multiple immunodetection platforms such as ELISA, Western Blotting, and Immunohistochemistry. Its broad compatibility is ensured through stringent quality control testing and high purity, making it ideal for researchers seeking dependable and accurate results in rat IgG (H+L) detection.


Robust Quality and Safety

Each batch undergoes rigorous testing to guarantee consistency and performance, with a purity above 95% and endotoxin levels kept under 2 EU/mg. Free from any harmful odor or coloration, and stabilized with sodium azide, this antibody is safe for use in standard laboratory environments and a reliable choice for research professionals.


Convenient Handling and Storage

The antibody is supplied in a ready-to-use liquid formulation, maintaining a physiological pH (7.2) and stabilized for optimal shelf life. For best results, store the product between 2-8C for short-term use, or at -20C for long-term storage, ensuring up to one year of dependable performance.

FAQs of Donkey Anti-Rat IgG (H+L):


Q: How should I properly store Donkey Anti-Rat IgG (H+L) for long-term use?

A: For optimal long-term stability, this antibody solution should be stored at -20C. If you plan to use it within a short timeframe, storage at 2-8C is suitable. Always avoid repeated freeze-thaw cycles to preserve antibody integrity.

Q: What are the recommended applications for this antibody?

A: Donkey Anti-Rat IgG (H+L) demonstrates excellent performance in ELISA, Western Blot, Immunohistochemistry, and other immunodetection assays focused on the detection of rat IgG heavy and light chains.

Q: When should I use the recommended dilution range of 1:500 to 1:5000?

A: The exact dilution depends on your assay type and conditions. Start with a 1:1000 dilution for initial optimization in ELISA or IHC, and adjust concentration as needed based on signal intensity and background.

Q: Where is this secondary antibody manufactured and supplied?

A: This product is distributed, imported, and supplied in the United States by a trusted supplier network, ensuring timely delivery and support for research institutes nationwide.

Q: What benefits does an unconjugated secondary antibody provide?

A: Using an unconjugated format offers flexibility for researchers to select a detection method of their choice, whether it be enzymatic, fluorescent, or other conjugates available upon request, to suit various experimental designs.

Q: How is the quality of this antibody controlled?

A: Each lot is rigorously tested in ELISA, Western Blot, and Immunohistochemistry applications. Purity is confirmed by SDS-PAGE and the endotoxin level is maintained below 2 EU/mg, supported by a certificate of analysis upon purchase.

Donkey Anti-Rat IgG (H+L)
Tell us about your requirement
product

Price:

Quantity
Select Unit

  • 50
  • 100
  • 200
  • 250
  • 500
  • 1000+
Additional detail
Mobile number

Email

More Products in Antisera Category

Rabbit Anti-Goat Whole Serum

Rabbit Anti-Goat Whole Serum

Price 8250 INR

Minimum Order Quantity : 1 Bottle

Purity(%) : 95% (IgG content by SDSPAGE)

Shelf Life : 12 months from date of manufacture (when properly stored)

Other Names : AntiGoat Immunoglobulin, Rabbit AntiGoat Antibody

Appearance : Clear Liquid

Get a Price/Quote
Goat Anti-Human Whole Serum

Goat Anti-Human Whole Serum

Price 8610 INR

Minimum Order Quantity : 1 Pack

Purity(%) : >95%

Shelf Life : 1224 months

Other Names : Goat AntiHuman IgG (Whole Serum)

Appearance : Liquid

Get a Price/Quote
Goat Anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG (H+L)

Price 3260 INR

Minimum Order Quantity : 1 Pack

Purity(%) : >95%

Shelf Life : 12 Months

Other Names : Goat AntiRabbit IgG (H+L)

Appearance : Liquid

Get a Price/Quote
Rabbit Anti-Human IgG, Fc fragment specific

Rabbit Anti-Human IgG, Fc fragment specific

Price 9820 INR

Minimum Order Quantity : 1 Pack

Purity(%) : > 95% (by SDSPAGE)

Shelf Life : 12 months

Other Names : AntiHuman IgG Fc (Rabbit)

Appearance : Liquid

Get a Price/Quote


Contact Details

    GST : 07BXSPD1989H1ZY

  • Plot No- D17/103, Rohini Sector 3, Near Ram Lila Park, Delhi - 110085, India
  • Customer Care Number---011-27511805
  • Mr Rambhajan Dwivedi (Proprietor)
  • Mobile : 08045816517
  • Phone : 08045816517
Send Inquiry Send Inquiry
Send SMS Send SMS
   
Call Me Call Me Free
  • trusted seller
  • Quick Links
    Our Products
    View All
    Back to top
    Tradeindia
    SR GROUP All Rights Reserved.(Terms of Use)
    Developed and Managed by Infocom Network Private Limited.