Goat Anti-Rabbit IgG (H+L)

3260 INR

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Product Details:

HS Code 3822
Molecular Weight Approximately 150 kDa
Poisonous No
Density 1 Gram per cubic centimeter(g/cm3)
Application Immunoassays, Western Blot, ELISA, Immunohistochemistry
Storage 2-8C for short term, -20C for long term
Smell Odorless
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Goat Anti-Rabbit IgG (H+L) Price And Quantity

Price 3260 INR
Price Range 3260.00 - 6500.00 INR
Minimum Order Quantity 1 Pack

Goat Anti-Rabbit IgG (H+L) Product Specifications

Poisonous No
Density 1 Gram per cubic centimeter(g/cm3)
Application Immunoassays, Western Blot, ELISA, Immunohistochemistry
Molecular Weight Approximately 150 kDa
HS Code 3822
Color Colorless to Light Yellow
Grade Research Grade
Purity(%) >95%
Shelf Life 12 Months
Classification Antibody
Other Names Goat Anti-Rabbit IgG (H+L)
Type Polyclonal Antibody
Chemical Name Goat Anti-Rabbit Immunoglobulin G (H+L)
Storage 2-8C for short term, -20C for long term
Smell Odorless
Physical Form Solution
Usage Secondary antibody for detection of rabbit IgG
Appearance Liquid
Ph Level 7.2-7.4

Product Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: 0.05% Sodium Azide

Versatile Polyclonal Antibody for Reliable Detection

This Goat Anti-Rabbit IgG (H+L) is engineered for researchers seeking a robust secondary antibody for the detection of rabbit IgG. With greater than 95% purity and a physical form ranging from colorless to light yellow, it adapts to different conjugation needs such as HRP or FITC, enhancing versatility across multiple immunoassays including Western Blot and ELISA.

Flexible Application and Simple Storage

Compatible with a range of methods, this antibody can be used in research settings for Western Blotting, ELISA, immunoassays, and immunohistochemistry. The product is provided in liquid form (with lyophilized versions available) and remains stable when stored at 28C for short-term or at 20C for extended periods, making it convenient for labs of all sizes.

Optimized Formulation and Safe Handling

Formulated in PBS with a neutral pH and containing 0.02% sodium azide as a preservative, this antibody is stable and easy to handle. It is shipped on ice packs and designed to be odorless, non-poisonous, and suitable for long-term storage, ensuring both safety and quality when handled in research environments.

FAQs of Goat Anti-Rabbit IgG (H+L):

Q: How should Goat Anti-Rabbit IgG (H+L) be stored to maintain optimal performance?

A: For short-term storage, keep the antibody at 28C. For long-term preservation, it is best stored at 20C. These conditions help retain antibody stability and extend shelf life up to 12 months.

Q: What are the recommended dilutions for using this antibody in Western Blot and ELISA applications?

A: For Western Blot, a dilution ratio of 1:10001:10000 is recommended. For ELISA, use a dilution between 1:200 and 1:1000. Exact dilutions might vary depending on specific experimental conditions.

Q: When is it beneficial to choose conjugated versus unconjugated Goat Anti-Rabbit IgG (H+L)?

A: Conjugated antibodies (e.g., with HRP or FITC) simplify detection steps and are ideal for direct signal visualization in immunoassays. Unconjugated forms are useful when a customized or multi-step detection system is required.

Q: Where is this antibody most commonly used in research?

A: This secondary antibody is widely utilized in research laboratories for immunoassays such as Western Blot, ELISA, and immunohistochemistry to detect rabbit IgG in samples.

Q: How does this product ensure specificity and quality in experimental applications?

A: This antibody is polyclonal and generated against purified rabbit IgG, offering high specificity. It is also purified to greater than 95%, minimizing cross-reactivity and ensuring reliable results in detection systems.

Q: What safety considerations are associated with this antibody?

A: The formulation contains 0.02% sodium azide as a preservative and should be handled with appropriate laboratory precautions. It is non-poisonous and odorless, designed for safe laboratory use, but strictly for research and not for diagnostic or therapeutic purposes.

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