Goat Anti-Mouse IgM, Chain Specific

10250 INR

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Product Details:

Grade Research Grade
Molecular Weight ~150 kDa
Usage Detection of mouse IgM ( chain) antibodies
Smell Odorless
Shelf Life 12 months
Molecular Formula Not applicable (protein mixture)
Physical Form Solution
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Goat Anti-Mouse IgM, Chain Specific Price And Quantity

Price 10250 INR
Price Range 10250.00 - 13600.00 INR
Minimum Order Quantity 1 Pack

Goat Anti-Mouse IgM, Chain Specific Product Specifications

Type Antibody
Appearance Liquid
Physical Form Solution
Poisonous No
Classification Polyclonal Antibody
Shelf Life 12 months
Structural Formula Protein Structure (IgG type)
Storage Store at 2-8C
Smell Odorless
Ph Level pH 7.2 0.2
Molecular Formula Not applicable (protein mixture)
Usage Detection of mouse IgM ( chain) antibodies
Purity(%) >=95% (by SDS-PAGE)
Density Gram per cubic centimeter(g/cm3)
Chemical Name Goat Anti-Mouse IgM, Chain Specific
Color Colorless to Pale Yellow
Other Names IgM Chain Goat Anti-Mouse
Application Immunological assays such as ELISA, western blot, immunohistochemistry
Grade Research Grade
HS Code 3822
Molecular Weight ~150 kDa

Product Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Superior Specificity for Mouse IgM Chain

This antibody is engineered to specifically recognize the chain of mouse IgM antibodies, ensuring accurate detection with minimal cross-reactivity to other mouse immunoglobulin isotypes. Its targeted performance streamlines analytical workflows in laboratory research.

Versatile Applications in Immunoassays

Suitable for ELISA, western blot, and immunohistochemistry, this polyclonal antibody provides reliable results across various immunological methodologies. Its broad usability supports diverse experimental setups for research and diagnostic studies.

High Purity and Quality Assurance

With a purity of at least 95% (as verified by SDS-PAGE) and endotoxin levels below 0.01 EU/g, this product assures consistent and reproducible outcomes. Its liquid, filtered formulation maintains integrity throughout its 12-month shelf life, provided it is stored at 28C.

FAQs of Goat Anti-Mouse IgM, Chain Specific:

Q: How should Goat Anti-Mouse IgM, Chain Specific be stored?

A: The antibody should be stored at 28C to maintain stability and efficacy. Under these conditions, its shelf life is up to 12 months.

Q: What is the recommended dilution range for this antibody in immunological assays?

A: Depending on the specific application, the suggested dilution is between 1:1,000 and 1:10,000. Optimization may be required based on assay type and sample conditions.

Q: When can this antibody be used in laboratory protocols?

A: Its suitable for use during the detection of mouse IgM antibodies in immunoassays such as ELISA, western blot, or immunohistochemistry, especially when high specificity for the chain is required.

Q: Where is this antibody most beneficial in research workflows?

A: This antibody is especially useful in academic, industrial, or clinical laboratories where precise and reliable identification of mouse IgM ( chain) is crucial for immunological studies or biomarker investigations.

Q: What advantages does this polyclonal antibody offer over monoclonal formats?

A: Polyclonal antibodies like this offer broader epitope recognition, enhancing sensitivity and robustness in detecting antigen variations. This can be especially advantageous in complex or heterogeneous samples.

Q: How is the specificity for mouse IgM ensured?

A: The antibody is generated using purified mouse IgM ( chain) as the immunogen, resulting in minimal cross-reactivity with other mouse immunoglobulins and maximizing assay specificity.

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