Rabbit Anti-Sheep IgG (H+L)

Rabbit Anti-Sheep IgG (H+L)

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Rabbit Anti-Sheep IgG (H+L)

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MOQ : 1 Bottle

Rabbit Anti-Sheep IgG (H+L) Specification

HS Code
38220090

Smell
Odorless

Storage
Store at 2-8°C, do not freeze

Other Names
Anti-Sheep Immunoglobulin G (Heavy & Light Chains)

Usage
Secondary antibody for detection of sheep IgG in multiple applications

Grade
Research Grade

Shape
Amorphous (liquid solution)

Molecular Weight
~150 kDa (monomeric IgG)

Density
1 Gram per cubic centimeter(g/cm3)

Ph Level
pH 7.2-7.4 (in PBS buffer)

Molecular Formula
Not applicable (protein mixture)

Physical Form
Solution

Poisonous
Non-poisonous

Application
Immunodetection, ELISA, Western Blot, IHC, Immunofluorescence

Shelf Life
12 months

Appearance
Liquid

Classification
Antibody, IgG Antiserum

Chemical Name
Rabbit Anti-Sheep IgG (H+L)

Type
Polyclonal Secondary Antibody

Purity(%)
>98% (by SDS-PAGE)

Color
Colorless to pale yellow

Preservative
0.09% Sodium Azide

Shipping Condition
Shipped on ice packs

Format
Liquid

Host
Rabbit

Species Reactivity
Sheep

Target Species
Sheep

Buffer
PBS (Phosphate Buffered Saline), pH 7.2, may contain stabilizers

Immunogen
Purified sheep IgG

Endotoxin Level
<1 EU/mg

Conjugation
None (unconjugated), check product variant for enzyme/dye labeling

Safety
For research use only, not for diagnostic or therapeutic use

QC Testing
SDS-PAGE, Immunoassay

Recommended Dilution
1:1,000 - 1:20,000 (depending on application)

Isotype
IgG (H+L)

About Rabbit Anti-Sheep IgG (H+L)

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule sheep IgG. It also reacts with the light chains of other sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Reliable Detection of Sheep IgG

This antibody offers robust and specific recognition of sheep IgG, making it ideal as a secondary antibody in various immunoassays. Its broad reactivity with both heavy and light chains ensures comprehensive detection in your experimental workflows, such as ELISA and Western blot.

Flexible Application Formats

Supplied in a colorless to pale yellow liquid solution, Rabbit Anti-Sheep IgG (H+L) suits a range of applications. Its recommended dilution (1:1,0001:20,000) allows you to tailor the usage according to assay sensitivity requirements, making it a versatile reagent for your research laboratory.

Quality Assurance and Safe Handling

With stringent quality control by SDS-PAGE and immunoassay, you receive a product with high purity for reliable results. The antibody is research grade, non-poisonous, and shipped on ice packs to maintain stability. Store at 28C and avoid freezing to preserve activity throughout its 12-month shelf life.

FAQs of Rabbit Anti-Sheep IgG (H+L):

Q: How is Rabbit Anti-Sheep IgG (H+L) used in laboratory applications?

A: This antibody serves as a secondary reagent to detect sheep IgG in various immunodetection methods, including ELISA, Western blot, immunohistochemistry, and immunofluorescence. Its broad reactivity with both heavy and light chains ensures accurate detection of target proteins.

Q: What is the recommended dilution range for this product?

A: The recommended dilution is between 1:1,000 and 1:20,000, depending on your specific assay and sensitivity requirements. It is advisable to optimize dilution for each application to obtain the best results.

Q: When should I consider using an enzyme- or dye-conjugated variant of this antibody?

A: If your assay requires direct detection, such as colorimetric or fluorescent readouts, consider selecting one of the labeled variants. For signal amplification via secondary detection, this unconjugated version is the appropriate choice.

Q: Where should I store the antibody for optimal stability and shelf life?

A: Store the antibody at 28C and avoid freezing. This ensures the product retains its stability and activity for up to 12 months from the date of receipt.

Q: What quality control measures have been taken to ensure product reliability?

A: Rabbit Anti-Sheep IgG (H+L) undergoes strict quality assessments, including SDS-PAGE for purity assurance (>98%) and validation through immunoassays for performance consistency.

Q: What are the main benefits of using this secondary antibody?

A: The antibody provides reliable, high-specificity detection of sheep IgG in diverse research applications, is validated for high purity, and comes in a ready-to-use liquid solution with a consistent shelf life when stored properly.

Rabbit Anti-Sheep IgG (H+L)

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