Hela Cell Line

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Product Details:

Physical Form Live cultured cells
Storage Liquid nitrogen or -80C
Shape Polyhedral, elongated
Grade Research grade
Density Gram per cubic centimeter(g/cm3)
Usage For in vitro research use only
Appearance Adherent cells in culture
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Hela Cell Line Price And Quantity

Hela Cell Line Product Specifications

Application Biomedical research, cancer studies, drug testing
Smell Odorless
Poisonous Non-poisonous
Shelf Life Long-term under proper storage
Purity(%) >99%
Chemical Name Hela Cell
Type Cell Line
Other Names Hela cells
Ph Level 7.2-7.4 in culture medium
Classification Human Epithelial Cell Line
HS Code 30029010
Color Whitish
Usage For in vitro research use only
Density Gram per cubic centimeter(g/cm3)
Appearance Adherent cells in culture
Physical Form Live cultured cells
Shape Polyhedral, elongated
Grade Research grade
Storage Liquid nitrogen or -80C

Hela Cell Line Trade Information

Main Domestic Market All India

Product Description

Hela Cell Line

Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, Hela cervical carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages) origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP) secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied. cell lines are available for double readout with both SEAP and Lucia luciferase

Exceptional Purity and Reliability

HeLa cell lines provided are authenticated, tested negative for mycoplasma and other contaminants, and maintain a purity above 99%. Each batch guarantees high cell viability after thawing, with a confluency of over 80% at harvest. These strict quality standards ensure reliable results for researchers using the cell line in critical scientific studies.

Flexible Applications in Research

With their robust adherence, rapid growth, and characteristic cobblestone-like morphology, HeLa cells are an essential tool in cancer biology, drug discovery, and other biomedical investigations. The ease of culture and proven genetic pedigree make them a preferred choice for in vitro experimentation across laboratories.

Safe Handling and Long-Term Storage

HeLa cells are classified as non-poisonous, research-grade material appropriate for BSL-2 conditions. Provided in cryopreservative media and shipped under ultra-cold temperatures, they have an extended shelf life when stored at -80C or in liquid nitrogen, supporting ongoing experimental needs.

FAQs of Hela Cell Line:

Q: How should I culture HeLa cells after thawing?

A: After thawing, gently transfer HeLa cells to a flask containing DMEM or MEM supplemented with 10% FBS. Ensure the medium is at 37C and culture in a humidified incubator with 5% CO2. Change medium regularly and passage cells when they reach 80% confluency.

Q: What biosafety precautions are required for handling HeLa cells?

A: HeLa cells should be handled in a BSL-2 laboratory setting, with standard aseptic techniques and appropriate personal protective equipment. Ensure all materials and waste are sterilized after use.

Q: When should I passage the HeLa cell line?

A: HeLa cells should be passaged when they reach 80% or greater confluency. Early passage is recommended to maintain optimal cell function and genetic integrity for experimental consistency.

Q: Where can I obtain authentication details for these cells?

A: An STR (Short Tandem Repeat) profile verifying cell line identity is available upon request, ensuring authenticity and reliability for your research applications.

Q: What are the recommended storage conditions for HeLa cells?

A: For long-term preservation, store HeLa cells in liquid nitrogen or at -80C. This ensures cell viability and genetic stability over extended periods.

Q: What are the main applications of the HeLa cell line?

A: HeLa cells are widely utilized in biomedical research, including cancer studies, drug testing, and general in vitro experimentation. Their robust growth and human origin make them invaluable for laboratory investigations.

Q: How does using HeLa cells benefit my research?

A: HeLa cells offer consistent growth, reliable genetic background, and high viability, enabling reproducible results in various scientific studies. Their broad acceptance and documented history facilitate compatibility with numerous experimental protocols.

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