Hek-293 Cell Line

About Hek-293 Cell Line

Hek-293 Cell Line 

Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, Hela cervical carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages) origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP) secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied.cell lines are available for double readout with both SEAP and Lucia luciferase

Applications of the HEK-293 Cell Line

The HEK-293 cell line is a preferred choice for applications such as gene expression analysis, recombinant protein production, viral propagation, and transfection experiments. Its robust growth and adaptability make it suitable for various in vitro research processes in biotechnology and molecular biology laboratories.


Handling and Storage Protocols

HEK-293 cells should always be handled under sterile conditions using BSL-2 safety protocols. For long-term viability, the cells must be stored in the vapor phase of liquid nitrogen. Cryopreservation uses 10% DMSO in FBS as a cryoprotectant, ensuring high survival rates after thawing.


Key Benefits of Using HEK-293 Cells

HEK-293 cells offer high transfection efficiency, exceptional adaptability between adherent and suspension growth forms, and reliable performance across research applications. Their purity, authentication, and contamination-free status streamline experimental workflows and ensure reproducible results.