Hek-293 Cell Line

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Product Details:

Usage In vitro research and biotechnology
Shape Epithelial-like
HS Code 3002
Molecular Weight Not applicable (cell line)
Physical Form Frozen vial or flask culture
Grade Research Grade
Structural Formula Not applicable (cell line)
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Hek-293 Cell Line Price And Quantity

Hek-293 Cell Line Product Specifications

Chemical Name HEK 293
Poisonous Non-toxic
Color Colorless to light yellow (in suspension culture medium)
Application Gene expression studies, recombinant protein production, viral propagation, transfection experiments
Storage Liquid nitrogen or -196C (cryopreservation)
Usage In vitro research and biotechnology
Shape Epithelial-like
HS Code 3002
Classification Cell Line
Appearance Adherent or suspension cells
Type Human Embryonic Kidney Cell Line
Ph Level 7.1 7.4 (culture condition)
Shelf Life Long-term in proper storage (cryopreserved)
Grade Research Grade
Purity(%) >95% viable cells
Structural Formula Not applicable (cell line)
Molecular Formula Not applicable (cell line)
Molecular Weight Not applicable (cell line)
Other Names Human Embryonic Kidney 293
Physical Form Frozen vial or flask culture
Smell Odorless

Hek-293 Cell Line Trade Information

Main Domestic Market All India

Product Description

Hek-293 Cell Line

Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, Hela cervical carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages) origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP) secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied.cell lines are available for double readout with both SEAP and Lucia luciferase

Applications of the HEK-293 Cell Line

The HEK-293 cell line is a preferred choice for applications such as gene expression analysis, recombinant protein production, viral propagation, and transfection experiments. Its robust growth and adaptability make it suitable for various in vitro research processes in biotechnology and molecular biology laboratories.

Handling and Storage Protocols

HEK-293 cells should always be handled under sterile conditions using BSL-2 safety protocols. For long-term viability, the cells must be stored in the vapor phase of liquid nitrogen. Cryopreservation uses 10% DMSO in FBS as a cryoprotectant, ensuring high survival rates after thawing.

Key Benefits of Using HEK-293 Cells

HEK-293 cells offer high transfection efficiency, exceptional adaptability between adherent and suspension growth forms, and reliable performance across research applications. Their purity, authentication, and contamination-free status streamline experimental workflows and ensure reproducible results.

FAQs of Hek-293 Cell Line:

Q: How should HEK-293 cells be stored to maintain their viability?

A: HEK-293 cells must be stored in the vapor phase of liquid nitrogen at or below -196C. This cryopreservation, with 10% DMSO in FBS, ensures their long-term viability and survival rates above 80% after thawing.

Q: What growth properties do HEK-293 cells exhibit?

A: HEK-293 cells can grow as either adherent or suspension cultures, depending on the variant used. Their epithelial-like morphology and reliable doubling time of approximately 34-36 hours make them versatile for diverse research applications.

Q: When are HEK-293 cells most commonly used in scientific research?

A: HEK-293 cells are extensively used for gene expression studies, recombinant protein production, viral propagation, and transfection experiments, especially in molecular biology and biotechnology laboratories.

Q: Where are HEK-293 cell lines sourced from?

A: HEK-293 cells originate from human embryonic kidney tissue, classified under Homo sapiens. They are provided by certified distributors, importers, suppliers, or traders in India for research applications.

Q: What process is recommended for thawing and culturing HEK-293 cells?

A: It is best to quickly thaw the frozen vial in a 37C water bath, transfer cells to pre-warmed DMEM or EMEM with 10% FBS, and immediately culture under sterile BSL-2 conditions to maximize cell survival and proliferation.

Q: Which precautions are necessary when handling HEK-293 cells?

A: HEK-293 cells should always be handled under sterile conditions using BSL-2 protocols to prevent contamination and ensure user safety, as well as to maintain experimental integrity.

Q: What are the main benefits of choosing the HEK-293 cell line for research?

A: HEK-293 cells offer high transfection efficiency, ease of culturing, and a reliable record of purity, authentication (STR profile available), and freedom from microbial contaminants, making them ideal for reproducible scientific applications.

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