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Raw Cell Line

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Raw Cell Line Specification

Taste
Odorless

Grade
Research Grade

Other Names
RAW 264.7

Shape
Round/Semi-adherent

Poisonous
Non-Poisonous

HS Code
38210000

Physical Form
Frozen Vial/Live Culture

Application
Immunology, Cytokine Studies, Inflammation Research

Smell
Odorless

Ph Level
7.2-7.4 (in culture medium)

Appearance
Adherent Cell Culture

Usage
In vitro cell-based assays, research and development purposes

Storage
Liquid Nitrogen (-196°C) for Frozen Vials; 37°C, 5% CO2 Incubator for Live Cultures

Shelf Life
2 years (frozen); Use immediately after thawing (live cultures)

Classification
Macrophage Cell Line

Chemical Name
RAW Cell Line

Type
Cell Line

Purity(%)
High

Color
Pale Yellow to Off-White

Species
Mus musculus (Mouse)

Cell Type
Monocyte/Macrophage Lineage

Mycoplasma Status
Tested, Negative

Growth Mode
Adherent

Biosafety Level
BSL-1

Freezing Medium
10% DMSO in FBS

Certificate Of Analysis
Available Upon Request

Shipment
Dry Ice (Frozen) / Ambient Temperature (Live)

Culture Medium
DMEM + 10% Fetal Bovine Serum

Contamination
Tested for Bacteria, Fungi, Mycoplasma

Doubling Time
~12-18 Hours

Authentication
STR Profile Provided

Viability
>90% post-thaw (frozen vial)

Raw Cell Line Trade Information

Payment Terms
Cash in Advance (CID), Cash Advance (CA)

Sample Available
Yes

Main Export Market(s)
Western Europe, South America, Eastern Europe, Middle East, Central America, Africa, Australia, Asia, North America

Main Domestic Market
All India

About Raw Cell Line

Raw Cell Line

Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, Hela cervical carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages) origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP) secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied.cell lines are available for double readout with both SEAP and Lucia luciferase

Reliable Source for Immunology and Inflammation Research

RAW Cell Line (RAW 264.7) is highly favored for studying immune mechanisms, cytokine production, and inflammation pathways. These mouse-derived macrophage cells exhibit robust adherence and high post-thaw viability, making them ideal for in vitro assays and advanced cellular research.

Strict Quality Controls and Proven Authentication

Each batch undergoes comprehensive testing for bacteria, fungi, and mycoplasma, ensuring consistent reliability. The cell lines STR profile is provided for authentication, while additional documentation such as the certificate of analysis is available to verify compliance and quality.

Easy Handling, Storage, and Shipment

Cells are prepared for convenience with options for frozen (shipped on dry ice) or live (ambient temperature) formats. Proper long-term storage in liquid nitrogen preserves frozen vials for up to two years. Live cultures must be reacclimated in a 37C, 5% CO2 incubator and used immediately after thawing.

FAQs of Raw Cell Line:

Q: How do I properly store and handle the RAW Cell Line after receiving it?

A: Frozen vials should be stored in liquid nitrogen at 196C for long-term preservation. Upon thawing, immediately transfer cells to DMEM with 10% FBS and incubate at 37C with 5% CO2. Live cultures must be handled promptly and maintained under appropriate growth conditions.

Q: What are the recommended culture conditions for RAW 264.7 cells?

A: Cultivate RAW 264.7 cells in DMEM containing 10% fetal bovine serum, in an adherent growth mode. Maintain them in a humidified incubator at 37C with 5% CO2. Regular passage is advised, as their doubling time ranges from 12 to 18 hours.

Q: When should I use RAW Cell Line for my experiments and research?

A: Utilize RAW 264.7 cells for in vitro assays involving immunology, cytokine studies, and inflammation research. Thanks to their monocyte/macrophage lineage, these cells offer reliable modeling for immune system mechanisms and cellular responses.

Q: Where are RAW Cell Line products tested for quality and authenticity?

A: RAW 264.7 cells undergo testing for contamination (bacteria, fungi, mycoplasma) and authentication via Short Tandem Repeat (STR) profiling prior to distribution. Distributors in India adhere to rigorous validation standards and provide documentation upon request.

Q: What is the process for ordering and shipping RAW 264.7 cell line in India?

A: Orders can be placed through authorized distributors, importers, or suppliers in India. Frozen vials ship on dry ice, while live cultures ship at ambient temperature to ensure cell integrity and high viability (>90%). A certificate of analysis is available upon request.

Q: How does using RAW Cell Line benefit my immunology or inflammation research?

A: RAW 264.7 cells provide a consistent, highly characterized platform for studying macrophage activity, cytokine production, and inflammatory processes. Their high purity, reproducibility, and reliable growth kinetics facilitate meaningful and reproducible experimental outcomes.

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