J-774 Cell line

J-774 Cell line

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J-774 Cell line

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J-774 Cell line Specification

Shape
Irregular, elongated or rounded

Grade
Research Grade

Poisonous
Non-poisonous

HS Code
30029030

Shelf Life
5 years from date of manufacture if properly stored

Storage
Store in liquid nitrogen

Smell
Odorless

Appearance
Adherent cells in suspension culture

Usage
For in vitro studies only, not for human or animal therapeutic use

Physical Form
Frozen vial

Application
Biomedical research, immunology, cancer research

Ph Level
7.2-7.4

Other Names
J774A.1

Classification
Monocytic, Macrophage

Chemical Name
J-774 Murine Macrophage Cell Line

Type
Cell line

Purity(%)
98

Color
Whitish to off-white cell pellet

Shipping Condition
Dry ice (frozen state)

Availability
Ready to ship

Cryopreservation Medium
10% DMSO in FBS

Authentication
STR profiled

Culture Medium
RPMI-1640 supplemented with 10% Fetal Bovine Serum

Doubling Time
Approximately 24 hours

Biosafety Level
BSL-1

Growth Mode
Adherent

Mycoplasma Status
Tested, negative

Organism
Mus musculus (mouse)

Tissue
Ascites

J-774 Cell line Trade Information

FOB Port
Delhi

Payment Terms
Cash in Advance (CID), Cash Advance (CA)

Supply Ability
25 Per Week

Delivery Time
1 Week

Sample Available
Yes

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Australia, Western Europe, Middle East, Central America, Africa, South America, Asia, Eastern Europe, North America

Main Domestic Market
All India

About J-774 Cell line

Cells are of various human (A549 lung carcinoma, HCT116 colon carcinoma, HEK293 human embryonic kidney cells, HeLa cervical

carcinoma, Jurkat T lymphocytes, Ramos B lymphocytes, THP-1 monocytes) or murine (B16 melanoma, J774 and RAW 264.7 macrophages)

origins. Our cell lines are stably transfected with reporter constructs encoding the secreted embryonic alkaline phosphatase (SEAP)

secreted Lucia luciferase gene downstream of a response element that is specific for the signaling pathway being studied.

cell lines are available for double readout with both SEAP and Lucia luciferase

Superior Reliability for Biomedical Research

J-774 Cell Line offers trusted performance for studies in immunology and cancer due to its robust monocytic macrophage characteristics and high authentication standards. Its tested, mycoplasma-negative status ensures consistent and uncontaminated results for researchers.

Easy Handling and Storage

Supplied in frozen vials, J-774 cells are simple to manage and store long-term at liquid nitrogen temperatures. With a shelf life of up to five years, researchers worldwide benefit from convenient delivery and sustained viability.

FAQs of J-774 Cell line:

Q: How should J-774 cells be stored upon receipt?

A: J-774 cells must be stored in liquid nitrogen immediately upon arrival to maintain cell viability and guarantee a shelf life of up to five years.

Q: What is the recommended culture medium for J-774 Cell Line maintenance?

A: RPMI-1640 supplemented with 10% Fetal Bovine Serum is the standard medium for optimal growth and maintenance of J-774 cells.

Q: When is the J-774 Murine Macrophage Cell Line ready for use?

A: J-774 cell vials are ready to use upon receipt and proper thawing. They can be cultured immediately for in vitro studies.

Q: Where is the J-774 cell line sourced and shipped from?

A: As a distributor, importer, and supplier in India, we ensure the J-774 cell line is available ready to ship and arrives under dry ice conditions to preserve integrity.

Q: What is the authentication process for J-774 cells?

A: J-774 cells undergo Short Tandem Repeat (STR) profiling to verify their identity, and they are confirmed free of mycoplasma contamination before shipment.

Q: How are the J-774 cells beneficial for research?

A: These murine macrophage cells are widely used in immunology and cancer research, providing reliable, non-poisonous, research-grade material for various biomedical applications.

Q: What is the recommended usage of the J-774 Cell Line?

A: J-774 cells are strictly intended for in vitro research use only and are not suitable for human or animal therapeutic purposes.

J-774 Cell line

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